Tsai C C, Chen C L, Hsu H C
Department of Pathology, National Taiwan University, Taiwan.
Hum Pathol. 1996 Mar;27(3):258-62. doi: 10.1016/s0046-8177(96)90066-0.
To elucidate the role of Epstein-Barr virus (EBV) in salivary gland carcinomas, 56 cases of carcinomas of major salivary glands were investigated. These included 14 mucoepidermoid carcinomas, 13 adenoid cystic carcinomas, seven malignant mixed tumors, four adenocarcinomas, four salivary duct carcinomas, two acinic cell carcinomas, two undifferentiated carcinomas without lymphoid stroma, seven lymphoepithelioma-like carcinomas (LELCs), two squamous cell carcinomas, and one small cell carcinoma. EBV transcripts were examined by in situ hybridization using digoxigenin-labeled oligonucleotide antisense probe for EBV-encoded RNA 1 (EBER1) on formalin-fixed paraffin-embedded tissue sections. EBER1 was detected in the malignant epithelial cells in all seven cases of LELC, but not in any of the other carcinomas and the neighboring normal salivary gland tissue. Because all the EBV-negative cases showed satisfactory labeling with the poly d(T) probe, the negative reaction with EBER1 was unlikely to be caused by poor RNA preservation in the tissues. The seven cases of LELC, which were histologically indistinguishable from undifferentiated nasopharyngeal carcinoma (NPC), had a disease-free 4-year survival rate of 85.7%. The results suggest that LELC of the salivary gland in Taiwanese Chinese may share similar EBV-related pathogenesis with that of NPC.
为阐明爱泼斯坦-巴尔病毒（EBV）在涎腺癌中的作用，对56例大涎腺癌病例进行了研究。这些病例包括14例黏液表皮样癌、13例腺样囊性癌、7例恶性混合瘤、4例腺癌、4例涎腺导管癌、2例腺泡细胞癌、2例无淋巴样间质的未分化癌、7例淋巴上皮瘤样癌（LELC）、2例鳞状细胞癌和1例小细胞癌。采用地高辛标记的EBV编码RNA 1（EBER1）寡核苷酸反义探针，通过原位杂交在福尔马林固定石蜡包埋组织切片上检测EBV转录本。在所有7例LELC的恶性上皮细胞中均检测到EBER1，但在其他任何癌组织及邻近正常涎腺组织中均未检测到。由于所有EBV阴性病例用聚d（T）探针均显示出满意的标记，因此EBER1的阴性反应不太可能是由组织中RNA保存不佳所致。这7例LELC在组织学上与未分化鼻咽癌（NPC）难以区分，其4年无病生存率为85.7%。结果表明，台湾华人涎腺的LELC可能与NPC具有相似的EBV相关发病机制。
