Lane D R, Joshi P, Grogan J B, Nie C H, Scott-Conner C E
Department of Surgery, University of Mississippi School of Medicine, Jackson, 52242-1086, USA.
Am Surg. 1996 Apr;62(4):259-62.
Prior studies regarding immune function in bile-duct ligated rats have illustrated blunted immune function. The present study measures natural killer (NK) cell activity. Rats underwent bile duct ligation (BDL) or sham celiotomy (SC) and were sacrificed at 1, 2, and 3 weeks after surgery. Ficoll-Hypaque density centrifugation was used to obtain a purified preparation of splenocytes. NK cell activity was determined by incubating varying concentrations of splenocytes with chromium-labelled YAC-1 tumor cells for 4 hours. Chromium release was measured by a gamma counter and expressed as per cent activity (compared with 100 per cent activity obtained by complete lysis with detergent). The experiments were repeated after preincubation in tissue culture flasks to remove an adherent cell population. NK cell activity was decreased at all spleen cell:target cell ratios studies at 1 week after BDL. At 2 weeks after BDL, NK cell activity was decreased at all but the lowest two concentrations; and 3 weeks after BDL, NK cell activity was decreased only at the highest concentrations used. Separation of an adherent cell fraction restored NK cell activity. This suppression in NK cell activity one week after BDL may account, in part, for the poor response of BDL animals to bacterial and immune challenge. Restoration of activity after removal of an adherent cell fraction suggests that macrophages may be at least partly responsible for this inhibition.
先前关于胆管结扎大鼠免疫功能的研究表明其免疫功能减弱。本研究检测自然杀伤（NK）细胞活性。对大鼠进行胆管结扎（BDL）或假剖腹手术（SC），并在术后1、2和3周处死。采用Ficoll-Hypaque密度梯度离心法获得纯化的脾细胞制剂。通过将不同浓度的脾细胞与铬标记的YAC-1肿瘤细胞孵育4小时来测定NK细胞活性。用γ计数器测量铬释放量，并以活性百分比表示（与用去污剂完全裂解获得的100%活性相比）。在组织培养瓶中预孵育以去除贴壁细胞群体后重复实验。在BDL后1周，所有研究的脾细胞与靶细胞比例下NK细胞活性均降低。在BDL后2周，除了最低的两个浓度外，所有浓度下NK细胞活性均降低；在BDL后3周，仅在所用的最高浓度下NK细胞活性降低。分离贴壁细胞部分可恢复NK细胞活性。BDL后1周NK细胞活性的这种抑制可能部分解释了BDL动物对细菌和免疫攻击反应不佳的原因。去除贴壁细胞部分后活性的恢复表明巨噬细胞可能至少部分负责这种抑制作用。
