Allavena P, Introna M, Mangioni C, Mantovani A
J Natl Cancer Inst. 1981 Aug;67(2):319-25.
Tumor-associated lymphocytes (TAL) were isolated from ascites ovarian tumors by stepwise application of density and velocity sedimentation on discontinuous Ficoll-Hypaque gradients and fetal bovine serum. TAL had low levels of natural killer (NK) activity compared to levels of peripheral blood lymphocytes (PBL) from the same patients or control subjects. When TAL were mixed with PBL, significant inhibition of NK activity was observed in 7 of 27 patients tested, with the suppression levels ranging from 14 to 60%. Inhibition of PBL NK activity was observed at the ratio of TAL to PBL of 1:1 or 2:1. Suppression of NK activity was detected with effector-to-target cell ratios ranging from 6:1 to 25:1 and at incubation time from 4 to 20 hours in the cytolysis assay. Tumor-associated macrophages from 6 patients were tested for suppression of NK activity. Only with 1 donor was a 17% inhibition observed at a ratio of macrophages of PBL of 1:1. Thus suppression by mature plastic adherent macrophages does not play a major role in the determination of the low levels of NK in human ascites ovarian tumors and the inhibitory activity of suppressor TAL, which had a minor contamination (less than 5%) with mononuclear phagocytes. When TAL from 1 patient with suppressive activity were passed through nylon wool, inhibition of NK activity was observed with both adherent and nonadherent cells.
通过在不连续的Ficoll-泛影葡胺梯度和胎牛血清上逐步应用密度和速度沉降法，从腹水卵巢肿瘤中分离出肿瘤相关淋巴细胞（TAL）。与来自相同患者或对照受试者的外周血淋巴细胞（PBL）水平相比，TAL的自然杀伤（NK）活性水平较低。当TAL与PBL混合时，在27例受试患者中有7例观察到NK活性受到显著抑制，抑制水平在14%至60%之间。在TAL与PBL的比例为1:1或2:1时观察到对PBL NK活性的抑制。在细胞溶解试验中，当效应细胞与靶细胞的比例为6:1至25:1以及孵育时间为4至20小时时检测到NK活性的抑制。对6例患者的肿瘤相关巨噬细胞进行了NK活性抑制测试。仅在1名供体中，当巨噬细胞与PBL的比例为1:1时观察到17%的抑制。因此，成熟的可塑性贴壁巨噬细胞的抑制作用在人类腹水卵巢肿瘤中NK水平较低的决定因素以及抑制性TAL的抑制活性中并不起主要作用，抑制性TAL仅受到单核吞噬细胞的少量污染（小于5%）。当将1例具有抑制活性患者的TAL通过尼龙毛柱时，贴壁细胞和非贴壁细胞均观察到NK活性的抑制。
