High-performance liquid chromatographic analysis of glycosaminoglycan-derived oligosaccharides.

High-performance liquid chromatography of glycosaminoglycan (GAG)-derived oligosaccharides has been employed for the structural analysis and measurement of hyaluronan, chondroitin sulphate, dermatan sulphate, keratan sulphate, heparan sulphate and heparin. Recent developments in the separation and detection of unsaturated disaccharides and oligosaccharides derived from GAGs by enzymatic or chemical degradation are reviewed.