丙氨酰 - tRNA合成酶识别过程中，来自tRNA（丙氨酸）相邻螺旋位点的G·U摆动碱基对的特定功能。
Specific function of a G.U wobble pair from an adjacent helical site in tRNA(Ala) during recognition by alanyl-tRNA synthetase.
作者信息
McClain W H, Gabriel K, Schneider J
机构信息
Department of Bacteriology, University of Wisconsin, Madison 53706-1567, USA.
出版信息
RNA. 1996 Feb;2(2):105-9.
Abstract
G.U wobble pairs are crucial to many examples of RNA-protein recognition. We previously concluded that the G.U wobble pair in the acceptor helix of Escherichia coli alanine tRNA (tRNA(Ala)) is recognized indirectly by alanyl-tRNA synthetase (AlaRS), although direct recognition may play some role. Our conclusion was based on the finding that amber suppressor tRNA Ala with G.U shifted to an adjacent helical site retained substantial but incomplete Ala acceptor function in vivo. Other researchers concluded that only direct recognition is operative. We report here a repeat of our original experiment using tRNA(Lys) instead of tRNA(Ala). We find, as in the original experiment, that a shifted G.U confers Ala acceptor activity. Moreover, the modified tRNA(Lys) was specific for Ala, corroborating our original conclusion and making it more compelling.
摘要
G·U摆动配对对于许多RNA-蛋白质识别的例子至关重要。我们之前得出结论，大肠杆菌丙氨酸tRNA（tRNA(Ala)）接受臂螺旋中的G·U摆动配对是由丙氨酰-tRNA合成酶（AlaRS）间接识别的，尽管直接识别可能也起一定作用。我们的结论基于这样一个发现，即带有G·U的琥珀抑制tRNA Ala转移到相邻螺旋位点后，在体内仍保留了相当但不完整的丙氨酸接受功能。其他研究人员得出结论，只有直接识别起作用。我们在此报告使用tRNA(Lys)而非tRNA(Ala)重复我们原来的实验。我们发现，与原来的实验一样，移位的G·U赋予了丙氨酸接受活性。此外，修饰后的tRNA(Lys)对丙氨酸具有特异性，证实了我们原来的结论并使其更具说服力。
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