Masson J M, Miller J H
Gene. 1986;47(2-3):179-83. doi: 10.1016/0378-1119(86)90061-2.
A synthetic promoter derived from the Escherichia coli lipoprotein promoter sequence was used to express synthetic suppressor tRNA genes in E. coli. These constructs, cloned into a plasmid of the pEMBL family, gave very high yields of suppressor tRNA in vivo.
一种源自大肠杆菌脂蛋白启动子序列的合成启动子被用于在大肠杆菌中表达合成抑制性tRNA基因。这些构建体被克隆到pEMBL家族的质粒中,在体内产生了非常高产量的抑制性tRNA。