Brüning G, Hattwig K, Mayer B
Department of Anatomy, Free University of Berlin, Königin-Luise-Strasse 15, D-14195 Berlin, Germany.
Cell Tissue Res. 1996 Apr;284(1):87-98. doi: 10.1007/s004410050569.
Neuronal nitric oxide synthase was located in various organs of the goldfish by NADPH-diaphorase histochemistry and immunohistochemistry. Positive cells were detected throughout the digestive tract. A particularly dense plexus of nitric-oxide-synthase-containing fibers was present at the opening of the pneumatic duct into the esophagus and at the intestinal sphincter separating the esophagus and the intestinal bulb. The nitroxergic innervation was mainly confined to the muscularis. The muscular layer of the swim bladder and of the pneumatic duct was densely equipped with stained neurons and fibers. In the heart, the majority of small neurons located at the sinu-atrial junction was found to be positive for nitric oxide synthase. The muscularis of the urinary duct was supplied by fibers originating from many intramural ganglia harboring intensely stained neurons. These results suggest that nitric oxide represents a widespread transmitter in the peripheral nervous system of teleost species.
通过NADPH黄递酶组织化学和免疫组织化学方法，在金鱼的各个器官中定位了神经元型一氧化氮合酶。在整个消化道均检测到阳性细胞。在鳔管通入食管的开口处以及分隔食管和肠球的肠括约肌处，存在特别密集的含一氧化氮合酶纤维丛。氮能神经支配主要局限于肌层。鳔和鳔管的肌层密布着染色的神经元和纤维。在心脏中，发现位于窦房结的大多数小神经元一氧化氮合酶呈阳性。输尿管肌层由许多含有染色强烈的神经元的壁内神经节发出的纤维供应。这些结果表明，一氧化氮是硬骨鱼类外周神经系统中一种广泛存在的递质。
