Umehara K, Kataoka K, Ogura T, Esumi H, Kashima K, Ibata Y, Okamura H
Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
Brain Res Bull. 1997;42(6):469-78. doi: 10.1016/s0361-9230(96)00374-7.
We investigated the localization of nitric oxide synthase in the pancreas of the dog in comparison to the rat by the methods of immunocytochemistry using antineuronal type nitric oxide synthase serum and histochemistry using NADPH-diaphorase activity. In both species, the most intense staining was observed in neuronal cell bodies and fibers in the pancreas and nitric oxide synthase immunoreactivity was completely colocalized with NADPH-diaphorase activity. However, there were differences of the distribution between the two species. In the dog pancreas, immuno- and NADPH-diaphorase-positive nerve fibers were numerous around pancreatic ducts and moderate around the arteries and the acini but few in the islets. In contrast, in the rat pancreas, immuno- and diaphorase-positive fibers were fewer around the pancreatic ducts and acini and more abundant in the islets. The expression ratio of NADPH-diaphorase in intrapancreatic ganglion cell bodies that were scattered in the interlobular connective tissue was low to moderate (28.1% in the right lobe, 49.5% in the left lobe) in the dog, while the ratio in rat pancreas was very high in both lobes of the pancreas (about 86%). Except for neuronal staining, weak NADPH-diaphorase-positive reactions were detected in the vascular endothelial cells of the pancreas in both species. In rat islet cells, weak neuronal type nitric oxide synthase immunoreactivity was observed; however, in dog islet cells, no immunoreactivity was detected. These results suggest that nitric oxide in the pancreas is derived from vascular endothelium and neuronal tissue in both species and that the neuronal nitrergic regulation of the exocrine and endocrine pancreas is different between the species.
我们采用抗神经元型一氧化氮合酶血清的免疫细胞化学方法以及利用NADPH-黄递酶活性的组织化学方法，对犬胰腺中一氧化氮合酶的定位进行了研究，并与大鼠进行了比较。在这两个物种中，胰腺的神经元细胞体和纤维中均观察到最强的染色，且一氧化氮合酶免疫反应性与NADPH-黄递酶活性完全共定位。然而，两个物种之间存在分布差异。在犬胰腺中，免疫和NADPH-黄递酶阳性神经纤维在胰管周围众多，在动脉和腺泡周围中等数量，但在胰岛中很少。相比之下，在大鼠胰腺中，免疫和黄递酶阳性纤维在胰管和腺泡周围较少，而在胰岛中更为丰富。在犬的胰腺中，散在于小叶间结缔组织中的胰腺内神经节细胞体中NADPH-黄递酶的表达率低至中等（右叶为28.1%，左叶为49.5%），而在大鼠胰腺的两个叶中该比率都非常高（约86%）。除神经元染色外，在两个物种的胰腺血管内皮细胞中均检测到微弱的NADPH-黄递酶阳性反应。在大鼠胰岛细胞中，观察到微弱的神经元型一氧化氮合酶免疫反应性；然而，在犬胰岛细胞中未检测到免疫反应性。这些结果表明，两个物种胰腺中的一氧化氮均来源于血管内皮和神经组织，并且外分泌和内分泌胰腺的神经元一氧化氮能调节在两个物种之间存在差异。
