Benzie I F
Department of Health Sciences, Hong Kong Polytechnic University, Hong Kong.
Clin Biochem. 1996 Apr;29(2):111-6. doi: 10.1016/0009-9120(95)02013-6.
To evaluate an automated enzyme linked, ferric-tripyridyltriazine spectrophotometric assay (EFTSA) for plasma ascorbic acid.
Using aqueous ascorbic acid solutions and plasma containing native and/or added ascorbic acid, the following were assessed: reaction kinetics, dose response relationships, recovery of added ascorbic acid, specificity, precision.
Performing the test on a Cobas Fara centrifugal analyser, the test is linear at least to 400 micromol/L; within-run CVs at 20, 50, 140, and 300 micromol/L ascorbic acid, in both pure aqueous solutions and in plasma, were <5.5%; 99-105% of added ascorbic acid (from 30-130 micromol/L) was recovered. The reaction of ascorbic acid is virtually instantaneous; other native antioxidants do not appear to interfere, and there is no interference by dehydroascorbic acid when readings are taken within a 15-60-s reaction time window.
EFTSA appears suitable for the routine measurement of ascorbic acid in plasma.
评估一种用于血浆中维生素C的自动化酶联、三吡啶三嗪铁分光光度法（EFTSA）。
使用抗坏血酸水溶液以及含有天然和/或添加抗坏血酸的血浆，评估以下各项：反应动力学、剂量反应关系、添加抗坏血酸的回收率、特异性、精密度。
在Cobas Fara离心分析仪上进行该测试，该测试至少在400微摩尔/升范围内呈线性；在纯水溶液和血浆中，抗坏血酸浓度为20、50、140和300微摩尔/升时，批内变异系数均<5.5%；添加的抗坏血酸（30 - 130微摩尔/升）回收率为99 - 105%。抗坏血酸的反应几乎是瞬间完成的；其他天然抗氧化剂似乎不产生干扰，并且在15 - 60秒的反应时间窗口内读取数据时，脱氢抗坏血酸也不产生干扰。
EFTSA似乎适用于血浆中维生素C的常规测量。
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