Lim Min Wen, Quan Tang Yin, Aroua Mohamed Kheireddine, Gew Lai Ti
Department of Biological Sciences, School of Medical and Life Sciences, Sunway University, No. 5, Jalan Universiti, Bandar Sunway, 47500 Petaling Jaya, Selangor, Malaysia.
School of Biosciences, Faculty of Health & Medical Sciences, Taylor's University, No. 1, Jalan Taylors, 47500 Subang Jaya, Selangor, Malaysia.
ACS Omega. 2024 Mar 14;9(12):14388-14405. doi: 10.1021/acsomega.4c00041. eCollection 2024 Mar 26.
Organic solvents are hazardous to human and environmental health. The emergence of interest in finding greener solvents to replace organic solvents has sparked a series of studies in the use of glycerol for extracting bioactive compounds from natural products. In this study, we will first identify the bioactive compounds of glycerol- and nonglycerol-based Thanaka () bark extracts using liquid chromatography-mass spectrometry profiles; then, we will determine their antioxidant capacity, free radical scavenging activity, and total phenolic and flavonoid contents. Thanaka bark powder was extracted using solvents, namely, ethanol (TKE), water (TKW), glycerol (TKG), glycerol/water (1:1, v/v) (TKGW), and glycerol/ethanol (1:1, v/v) (TKGE). Among the five extracts, the extract of TKG has the highest number of bioactive compounds, as well as the highest total flavonoid content. TKGE possessed the highest total phenolic content and highest antioxidant activity shown in azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and ferric-reducing antioxidant power assays among the five extracts. Overall, glycerol has better efficiency in extracting bioactive compounds from Thanaka bark as compared to ethanol and water. Hence, from the phytochemical content and antioxidant properties of Thanaka extracts, we conclude that glycerol is a good green solvent alternative to replace organic solvents.
有机溶剂对人体和环境健康有害。寻找更环保的溶剂来替代有机溶剂的兴趣促使人们开展了一系列关于使用甘油从天然产物中提取生物活性化合物的研究。在本研究中，我们将首先使用液相色谱 - 质谱图谱鉴定基于甘油和非甘油的楝树皮提取物中的生物活性化合物；然后，我们将测定它们的抗氧化能力、自由基清除活性以及总酚和黄酮含量。使用乙醇（TKE）、水（TKW）、甘油（TKG）、甘油/水（1:1，v/v）（TKGW）和甘油/乙醇（1:1，v/v）（TKGE）等溶剂提取楝树皮粉末。在这五种提取物中，TKG提取物的生物活性化合物数量最多，总黄酮含量也最高。在五种提取物中，TKGE在偶氮二（3 - 乙基苯并噻唑啉 - 6 - 磺酸）和铁还原抗氧化能力测定中表现出最高的总酚含量和最高的抗氧化活性。总体而言，与乙醇和水相比，甘油从楝树皮中提取生物活性化合物的效率更高。因此，从楝树提取物的植物化学成分和抗氧化特性来看，我们得出结论，甘油是替代有机溶剂的良好绿色溶剂选择。
