Sapats S I, Ashton F, Wright P J, Ignjatovic J
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville 3052, Australia.
J Gen Virol. 1996 Mar;77 ( Pt 3):413-8. doi: 10.1099/0022-1317-77-3-413.
Sequencing of the S1 genes of nine Australian strains of infectious bronchitis virus (IBV) identified two genotypically distinct groups of strains. The strains Vic S, V5/90, N1/62, N3/62, N9/74, and N2/75 comprised group I, sharing 80.7-98.3% identity in their deduced amino acid sequences. All group I strains were able to replicate in the trachea and kidney but only four strains, Vic S, N1/62, N9/74, and N2/75, were nephropathogenic, the latter three causing mortalities ranging from 32 to 96%. Group II contained strains N1/88, Q3/88 and V18/91 which only replicated in the trachea, inducing no mortalities. These viruses showed 72.3-92.8% amino acid identity to each other and only 53.8-61.7% identity to viruses of the first group. They were also distinct from the Massachusetts 41 and D1466 strains (47.5-55.7% amino acid identity). Thus N1/88, Q3/88 and V18/91 form a new group of viruses which are genotypically distinct from all previously characterized IBV strains. No definite correlations were established between the S1 amino acid sequences and the nephropathogenicity of strains.
对9株澳大利亚传染性支气管炎病毒（IBV）的S1基因进行测序后，鉴定出两个基因型不同的毒株组。Vic S、V5/90、N1/62、N3/62、N9/74和N2/75毒株组成第一组，其推导的氨基酸序列具有80.7 - 98.3%的同一性。所有第一组毒株都能在气管和肾脏中复制，但只有4株，即Vic S、N1/62、N9/74和N2/75具有肾致病性，后三株导致的死亡率在32%至96%之间。第二组包含N1/88、Q3/88和V18/91毒株，它们仅在气管中复制，不引起死亡。这些病毒彼此之间的氨基酸同一性为72.3 - 92.8%，与第一组病毒的同一性仅为53.8 - 61.7%。它们也与马萨诸塞州41和D1466毒株不同（氨基酸同一性为47.5 - 55.7%）。因此，N1/88、Q3/88和V18/91形成了一组新的病毒，其基因型与所有先前鉴定的IBV毒株不同。S1氨基酸序列与毒株的肾致病性之间未建立明确的相关性。
