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对粗糙脉孢菌中乙酸盐诱导型启动子进行凝胶迁移率变动扫描，发现了一种共同的共诱导DNA结合蛋白。

Gel mobility shift scanning of the acetate-inducible promoters from Neurospora crassa reveals a common co-inducible DNA-binding protein.

作者信息

Mizote T, Bibbins M, Sheffield P J, Connerton I F

机构信息

Department of Protein Engineering, Institute of Food Research, Reading, United Kingdom.

出版信息

Mol Gen Genet. 1996 Mar 7;250(4):421-7. doi: 10.1007/BF02174030.

DOI:10.1007/BF02174030
PMID:8602159
Abstract

The promoter regions of four acetate-inducible genes of Neurospora crassa, acu-3, acu-5, acu-8 and acu-9, have been sequenced. Using a scanning gel mobility shift assay particular DNA regions in each promoter have been shown specifically to bind partially purified protein extracted from acetate-induced mycelia. The protein-binding regions so defined have common sequence motifs, elements of which are similar to those required for acetate induction in aspergillus nidulans.

摘要

粗糙脉孢菌的四个乙酸盐诱导型基因acu - 3、acu - 5、acu - 8和acu - 9的启动子区域已被测序。使用扫描凝胶迁移率变动分析，已表明每个启动子中的特定DNA区域能特异性结合从乙酸盐诱导的菌丝体中提取的部分纯化蛋白。如此确定的蛋白结合区域具有共同的序列基序，其元件与构巢曲霉中乙酸盐诱导所需的元件相似。

相似文献

1
Gel mobility shift scanning of the acetate-inducible promoters from Neurospora crassa reveals a common co-inducible DNA-binding protein.对粗糙脉孢菌中乙酸盐诱导型启动子进行凝胶迁移率变动扫描，发现了一种共同的共诱导DNA结合蛋白。
Mol Gen Genet. 1996 Mar 7;250(4):421-7. doi: 10.1007/BF02174030.
2
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本文引用的文献

1
Specific binding sites in the alcR and alcA promoters of the ethanol regulon for the CREA repressor mediating carbon catabolite repression in Aspergillus nidulans.构巢曲霉中乙醇调节子的alcR和alcA启动子上的特异性结合位点，用于CREA阻遏物介导碳分解代谢物阻遏。
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2
A cis-acting region required for the regulated expression of grg-1, a Neurospora glucose-repressible gene. Two regulatory sites (CRE and NRS) are required to repress grg-1 expression.一种顺式作用区域，是粗糙脉孢菌葡萄糖可阻遏基因grg-1的调控表达所必需的。需要两个调控位点（CRE和NRS）来抑制grg-1的表达。
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3
Sequential gel mobility shift scanning of 5' upstream sequences of the Neurospora crassa am (GDH) gene.
粗糙脉孢菌am（GDH）基因5'上游序列的连续凝胶迁移率变动扫描
Mol Gen Genet. 1994 Feb;242(4):399-403. doi: 10.1007/BF00281789.
4
The two-way selection of mutants and revertants in respect of acetate utilization and resistance to fluoro-acetate in Aspergillus nidulans.构巢曲霉在醋酸利用和对氟乙酸抗性方面突变体与回复突变体的双向选择。
Genet Res. 1965 Nov;6(3):317-29. doi: 10.1017/s0016672300004213.
5
Acetate-nonutilizing mutants of Neurospora rassa. II. Biochemical deficiencies and the roles of certain enzymes.粗糙脉孢菌的乙酸盐非利用突变体。II. 生化缺陷及某些酶的作用
J Bacteriol. 1968 Mar;95(3):1063-8. doi: 10.1128/jb.95.3.1063-1068.1968.
6
Acetate-onutilizing mutants of Neurospora crassa. I. Mutant isolation, complementation studies, and linkage relationships.粗糙脉孢菌利用醋酸盐的突变体。I. 突变体的分离、互补研究及连锁关系。
J Bacteriol. 1968 Mar;95(3):1056-62. doi: 10.1128/jb.95.3.1056-1062.1968.
7
The role and control of the glyoxylate cycle in Escherichia coli.乙醛酸循环在大肠杆菌中的作用及调控
Biochem J. 1966 Apr;99(1):1-11. doi: 10.1042/bj0990001.
8
Binding of a nuclear factor to a regulatory sequence in the promoter of the mouse H-2Kb class I major histocompatibility gene.一种核因子与小鼠H-2Kb I类主要组织相容性基因启动子中的调控序列的结合。
Mol Cell Biol. 1987 Jan;7(1):305-13. doi: 10.1128/mcb.7.1.305-313.1987.
9
The amdS gene of Aspergillus nidulans: control by multiple regulatory signals.构巢曲霉的amdS基因：受多种调控信号的控制
Bioessays. 1986 Sep;5(3):123-8. doi: 10.1002/bies.950050308.
10
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Methods Enzymol. 1987;152:721-35. doi: 10.1016/0076-6879(87)52076-6.