Chen H L, Chen Y C, Chen D S
Hepatitis Research Center, National Taiwan University Hospital, Taipei.
Cancer Genet Cytogenet. 1996 Feb;86(2):102-6. doi: 10.1016/0165-4608(95)00186-7.
To investigate the chromosomal changes in hepatocellular carcinoma in humans, metaphase chromosomes obtained directly from short-term culture of resected human primary hepatocellular carcinoma tissues were studied in seven patients. Among the five successfully karyotyped cases, cytogenetic analysis showed highly different and complex chromosomal changes in the cancer tissue in four patients. Structural aberrations of chromosome 1 and deletion of 1p with breakpoints at p22 or p32 were the most common abnormalities, and were found in the four successfully analyzed cases. Loss of 1p may be important in hepatocarcinogenesis, and warrants further characterization by molecular genetic analysis.
为了研究人类肝细胞癌中的染色体变化，对7例患者经手术切除的原发性肝细胞癌组织进行短期培养后直接获取的中期染色体进行了研究。在5例成功进行核型分析的病例中，细胞遗传学分析显示4例患者癌组织中的染色体变化高度不同且复杂。1号染色体的结构畸变以及1p在p22或p32处断点的缺失是最常见的异常情况，在4例成功分析的病例中均有发现。1p缺失在肝癌发生过程中可能具有重要意义，值得通过分子遗传学分析作进一步鉴定。
