Ingster O, Hamon-Benais C, Couturier-Turpin M H, Guguen-Guillouzo C, Lucas J, Bernheim A, Feldmann G
Laboratoire de Biologie Cellulaire, Inserm U 327, Faculté de Medecine Xavier Bichat, Université Paris 7 Denis Diderot, France.
Ann Genet. 1996;39(3):123-8.
Cytogenetic analysis of a new human hepatoma cell line BC2 was performed with conventional cytogenetic techniques and fluorescent in situ hybridization. Numerical and structural abnormalities were observed by conventional cytogenetics for chromosomes 1, 2, 4, 7, 8, 9, 10, 11, 15, 17 and 20. Chromosome painting allowed to specify the translocation of chromosome 1, and to characterize 3 markers from chromosome 8 and one marker from 9, which were unrecognizable by conventional techniques. Comparison of chromosome 1 abnormalities with those reported in the literature for other human hepatoma cell lines showed that structural abnormalities of chromosome 1 were present in different regions of this chromosome. A review of the literature was done, and the results discussed, suggesting that alterations of chromosome 1 may be important in hepatocarcinogenesis.
采用传统细胞遗传学技术和荧光原位杂交技术对一种新的人肝癌细胞系BC2进行了细胞遗传学分析。通过传统细胞遗传学方法观察到1号、2号、4号、7号、8号、9号、10号、11号、15号、17号和20号染色体存在数目和结构异常。染色体描绘技术能够明确1号染色体的易位情况，并鉴定出8号染色体的3个标记和9号染色体的1个标记，而这些标记用传统技术无法识别。将1号染色体异常情况与文献中报道的其他人肝癌细胞系进行比较，结果显示1号染色体的结构异常存在于该染色体的不同区域。本文进行了文献综述并讨论了结果，提示1号染色体的改变可能在肝癌发生过程中具有重要作用。
