Su S B, Mukaida N, Matsushima K
Department of Pharmacology, Cancer Research Institute, Kanazawa University, Ishikawa, Japan.
J Leukoc Biol. 1996 Mar;59(3):420-6. doi: 10.1002/jlb.59.3.420.
Freshly isolated rabbit platelets contained a substantial amount of intracellular interleukin-8 (IL-8) as revealed by immunohistochemistry and Western blotting analysis. Thrombin stimulation at room temperature induced platelets to secrete IL-8 rapidly, and the maximal secretion was observed within 10 min after the activations. Secreted IL-8 possessed neutrophil chemotactic activity that was neutralized by a specific neutralizing monoclonal antibody to rabbit IL-8 but not a control one. These results suggest that rabbit platelets store biologically active IL-8 intracellularly and secrete it rapidly upon activation. This study implies that platelets may be a pivotal participant in inflammatory reactions in rabbits through producing cytokines and chemokines, including IL-8.
