Kitada K, Inoue M
Department of Preventive Dentistry, Kagoshima University Dental School, Japan.
Oral Microbiol Immunol. 1996 Feb;11(1):22-8. doi: 10.1111/j.1399-302x.1996.tb00332.x.
Carbohydrate antigens of the serotype k/Lancefield group G "Streptococcus milleri" were extracted by autoclaving whole cells of the type k reference strain Streptococcus anginosus K214-2K. The type k and group G antigen molecules are separated from each other and partially purified by a DEAE-Sephadex A25 column chromatography followed by a Sephadex G-100 gel filtration. In the double diffusion and the immunoelectrophoresis, the type k and group G antigen preparations obtained yielded single bands with their homologous antisera respectively. The type k antigen preparation contained principally glycerol, rhamnose, ribitol, galactose and glucose in a molar ratio of 0.54:1.20:0.43:0.33:1.00. The quantitative precipitin inhibition test indicated that beta(1-6)glucosyl-glucose (gentiobiose) sequence played a major role in immunodeterminant structure. Thus, the type k antigen of "S. milleri" appears to be a new carbohydrate type antigen that is chemically different from any Ottens type antigen. In contrast, the group G antigen preparation contained high proportion of rhamnose in addition to glucose, N-acetylglucosamine and N-acetylgalactosamine in a molar ratio of 6.45:1.00:0.24:1.15, and the rhamnose residue was involved in the immunodominant epitope, being in good agreement with the previously proposed chemical structure of the group antigen.
通过对血清型k/兰斯菲尔德G群“米勒链球菌”的全细胞进行高压灭菌，提取其碳水化合物抗原。参考菌株咽峡炎链球菌K214 - 2K的k型抗原分子与G群抗原分子通过DEAE - 葡聚糖A25柱色谱法分离，随后进行葡聚糖G - 100凝胶过滤，实现部分纯化。在双向扩散和免疫电泳中，获得的k型和G群抗原制剂分别与它们的同源抗血清产生单一条带。k型抗原制剂主要含有甘油、鼠李糖、核糖醇、半乳糖和葡萄糖，摩尔比为0.54:1.20:0.43:0.33:1.00。定量沉淀抑制试验表明，β(1 - 6)葡糖基 - 葡萄糖（龙胆二糖）序列在免疫决定簇结构中起主要作用。因此，“米勒链球菌”的k型抗原似乎是一种新的碳水化合物型抗原，在化学上与任何奥滕斯型抗原都不同。相比之下，G群抗原制剂除了含有葡萄糖、N - 乙酰葡糖胺和N - 乙酰半乳糖胺外，还含有高比例的鼠李糖，摩尔比为6.45:1.00:0.24:1.15，鼠李糖残基参与免疫显性表位，这与先前提出的群抗原化学结构一致。
