Konagawa R, Yakushiji T, Inoue M
Department of Preventive Dentistry, Kagoshima University Dental School, Japan.
Zentralbl Bakteriol. 1990 Oct;274(1):40-9. doi: 10.1016/s0934-8840(11)80973-4.
Type-specific carbohydrate antigen of the serotype i "Streptococcus milleri" was extracted with trichloroacetic acid from purified cell walls of the type strain K39K. The extracts were then purified by chromatography on DEAE-Sephadex A-25 and Sephadex G-100 columns. The purified serotype i carbohydrate antigen produced a single precipitin band against its homologous type-specific antiserum, which fused with the band produced by the autoclaved extract of the type strain cells. The serotype i antigen was a polysaccharide composed of rhamnose, galactose and glucose in a molar ratio of 1.6:6.8:1.0. The quantitative precipitin inhibition test with various haptenic sugars showed that galactose as well as lactose produced the greatest inhibition, which suggested that a galactose in terminal beta linkage is the immunodeterminant of the serotype i-specific antigen. Galactose was detected in the autoclaved extracts from cells of all the 15 serotype i strains tested.
用三氯乙酸从i型“米勒链球菌”模式菌株K39K的纯化细胞壁中提取i型特异性碳水化合物抗原。提取物随后通过在DEAE-葡聚糖A-25和葡聚糖G-100柱上进行色谱法纯化。纯化的i型碳水化合物抗原针对其同源的i型特异性抗血清产生单一沉淀带，该带与模式菌株细胞的高压灭菌提取物产生的带融合。i型抗原是一种多糖，由鼠李糖、半乳糖和葡萄糖以1.6:6.8:1.0的摩尔比组成。用各种半抗原糖进行的定量沉淀抑制试验表明，半乳糖以及乳糖产生的抑制作用最大，这表明末端β键连接的半乳糖是i型特异性抗原的免疫决定簇。在测试的所有15株i型菌株的细胞高压灭菌提取物中均检测到半乳糖。
