Inoue M, Yakushiji T, Konagawa R
Department of Preventive Dentistry, Kagoshima University Dental School, Japan.
Oral Microbiol Immunol. 1991 Oct;6(5):295-8. doi: 10.1111/j.1399-302x.1991.tb00496.x.
Cell wall carbohydrate antigen of the serotype g "Streptococcus milleri" was extracted with cold trichloroacetic acid from purified cell walls of the type strain K1K. The extracts were then purified by a DEAE-Sephadex A-25 column, followed by a Sephadex G-100 column. The immunoelectrophoresis revealed that the serotype g carbohydrate antigen preparation displayed a single precipitin band against the crude anti-K1K serum. The purified type g antigen consisted of rhamnose, galactose, glucose and galactosamine in a ratio of 1.3:3.8:1.0:2.5. The quantitative precipitin inhibition test with various haptens indicated that galactosamine is a major immunodeterminant of the type g-specific antigen.
用冷三氯乙酸从血清型g“米勒链球菌”模式菌株K1K的纯化细胞壁中提取细胞壁碳水化合物抗原。提取物随后通过DEAE-葡聚糖A-25柱进行纯化，接着通过葡聚糖G-100柱进行纯化。免疫电泳显示，血清型g碳水化合物抗原制剂针对粗制抗K1K血清呈现出一条单一的沉淀带。纯化的g型抗原由鼠李糖、半乳糖、葡萄糖和氨基半乳糖按1.3:3.8:1.0:2.5的比例组成。用各种半抗原进行的定量沉淀抑制试验表明，氨基半乳糖是g型特异性抗原的主要免疫决定簇。
