Yakushiji T, Inoue M, Koga T
Department of Preventive Dentistry, Kagoshima University Dental School, Japan.
Infect Immun. 1988 Sep;56(9):2264-9. doi: 10.1128/iai.56.9.2264-2269.1988.
The type-specific antigen of serotype b Streptococcus milleri was extracted with trichloroacetic acid from a purified cell wall preparation of the strain NCTC 10708 and then purified on a DEAE-Sephadex A-25 column, followed by a Sephadex G-100 column. The antigen was composed of rhamnose and glucose in a molar ratio of 1.7:1.0, with a trace of galactosamine (0.1). The quantitative precipitin inhibition test with various haptenic sugars showed that rhamnose gave the greatest inhibition, whereas glucose and its related carbohydrates were less effective. The major carbohydrate components of the Rantz-Randall extracts from cells of all four serotype b strains tested were also rhamnose and glucose. These results suggest that rhamnose is structurally involved in the immunodeterminant of the serotype b-specific antigen of oral S. milleri.
用三氯乙酸从菌株NCTC 10708的纯化细胞壁制剂中提取b型米勒链球菌的型特异性抗原，然后在DEAE-葡聚糖A-25柱上进行纯化，接着在葡聚糖G-100柱上纯化。该抗原由鼠李糖和葡萄糖组成，摩尔比为1.7:1.0，含有微量的半乳糖胺（0.1）。用各种半抗原糖进行的定量沉淀抑制试验表明，鼠李糖的抑制作用最大，而葡萄糖及其相关碳水化合物的效果较差。所测试的所有四种b型菌株细胞的兰茨-兰德尔提取物的主要碳水化合物成分也是鼠李糖和葡萄糖。这些结果表明，鼠李糖在口腔米勒链球菌b型特异性抗原的免疫决定簇结构中起作用。
