Palfi V, Christensen L S
National Veterinary Laboratory, Copenhagen N, Denmark.
Vet Microbiol. 1995 Nov;47(1-2):199-204. doi: 10.1016/0378-1135(95)00053-d.
Twenty-five strains of equine herpesvirus 1 (EHV-1) and one strain of equine herpesvirus 4 (EHV-4) isolated from material from various clinical cases in Denmark, together with reference EHV-1 and EHV-4 strains, were compared by restriction fragment pattern (RFP) analysis and inoculation of baby mice. The RFP analyses revealed that all EHV-1 strains belonged to genome type Ip. Four fetal isolates exhibited genomic characteristics that have been suggested as specific markers of the attenuated strain Rac H, widely used as a live vaccine. As the use of five vaccines against EHV-1 and EHV-4 has never been allowed in Denmark, it is assumed that Rac H derivatives have been acquired from visiting horses and thus are now circulating in the horse population. Baby mice inoculation revealed that four biotypes could be distinguished on the basis of pathogenicity. However, no strict correlation with pathogenicity in the natural host was seen.
从丹麦不同临床病例材料中分离出的25株马疱疹病毒1型（EHV-1）和1株马疱疹病毒4型（EHV-4），与EHV-1和EHV-4参考毒株一起，通过限制性片段图谱（RFP）分析和接种乳鼠进行了比较。RFP分析表明，所有EHV-1毒株均属于基因组类型Ip。4株胎儿分离株表现出的基因组特征被认为是广泛用作活疫苗的减毒株Rac H的特异性标记。由于丹麦从未允许使用5种针对EHV-1和EHV-4的疫苗，因此推测Rac H衍生物是从来访马匹中获得的，目前正在马群中传播。乳鼠接种表明，根据致病性可区分出4种生物型。然而，未观察到与天然宿主致病性的严格相关性。
