Vanrompay D, Mast J, Ducatelle R, Haesebrouck F, Goddeeris B
Department of Avian Medicine and Pathology, Gent, Belgium.
Vet Microbiol. 1995 Dec;47(3-4):245-56. doi: 10.1016/0378-1135(95)00125-5.
At 7 days of age, 4 groups, each of twenty specific pathogen free turkeys kept in isolation units were inoculated by aerosol with the Texas Turkey strain (avian Chlamydia psittaci serovar D), strain 92/1293 (avian Chlamydia psittaci serovar D), strain 84/55 (avian Chlamydia psittaci serovar A) or strain 89/1326 (avian Chlamydia psittaci serovar B). A fifth group of 4 specific pathogen free turkeys were sham inoculated controls. At daily intervals for 10 days and then twice weekly up to 34 days post infection, one bird in each group was killed and the target tissues and cells for replication and the sequence of events of serovar A, B and D infections was examined. In these turkeys, the primary site of replication was the respiratory tract. Chlamydial replication could be detected in the respiratory tract on day 1 post inoculation (p.i.) for group A, on day 3 p.i. for group B and on day 1 to 2 p.i. for groups D1 and D2. Subsequently, there was chlamydaemia and localisation in the digestive tract, in one or more parenchymatous organs, in the pericardium and in the conjunctivae. Specific immunoperoxidase staining revealed chamydiae in these organs in epithelial cells and in monomorphonuclear cells in all infected groups. The monomorphonuclear cells were identified as macrophages by double immunofluorescence staining. Chlamydiae were present in the same tissues for serovars A and D, but could not be demonstrated in proventriculus, duodenum, pancreas, ovaries and testes for serovar B. Furthermore, the intensity of replication was similar for all serovars. However, for serovar B in comparison with the other serovars, the bacteria appeared in most tissues 1 to 6 days later and the maximal replication in these tissues occurred 3 to 4 days later.
7日龄时，将4组、每组20只饲养在隔离单元中的无特定病原体火鸡通过气溶胶接种德州火鸡株（禽鹦鹉热衣原体D血清型）、92/1293株（禽鹦鹉热衣原体D血清型）、84/55株（禽鹦鹉热衣原体A血清型）或89/1326株（禽鹦鹉热衣原体B血清型）。第五组4只无特定病原体火鸡作为假接种对照。在感染后10天内每天、然后直至感染后34天每周两次，每组处死1只火鸡，检查用于复制的靶组织和细胞以及A、B和D血清型感染的事件顺序。在这些火鸡中，复制的主要部位是呼吸道。接种后第1天（感染后）在A组呼吸道中可检测到衣原体复制，B组在感染后第3天，D1组和D2组在感染后第1至2天。随后，出现衣原体血症，并定位于消化道、一个或多个实质器官、心包和结膜。特异性免疫过氧化物酶染色显示，所有感染组的这些器官的上皮细胞和单核细胞中有衣原体。通过双重免疫荧光染色将单核细胞鉴定为巨噬细胞。血清型A和D的衣原体存在于相同组织中，但血清型B在前胃、十二指肠、胰腺、卵巢和睾丸中未检测到。此外，所有血清型的复制强度相似。然而，与其他血清型相比，血清型B的细菌在大多数组织中出现的时间晚1至6天，并且在这些组织中的最大复制发生的时间晚3至4天。
