Zhu L, Kim K, Domenico D R, Appert H E, Howard J M
Department of Pathology, Medical College of Ohio, Toledo 43606, USA.
J Surg Oncol. 1996 Feb;61(2):100-5. doi: 10.1002/(SICI)1096-9098(199602)61:2<100::AID-JSO3>3.0.CO;2-G.
Oncogenes, tumor suppressor genes, and growth factors are being explored as to their role in the initiation and progression of most neoplasms, but little information exists on the expression of oncoproteins or growth factors in adenocarcinoma of the duodenum or ampulla of Vater. This report covers expressions of p53, c-neu, TGF-alpha, CEA, and EMA in duodenal adenocarcinoma and ampullary adenocarcinoma, as well as correlations between expressions and tumor stage, histological grade and patient survival. The expression of p53, c-neu, TGF-alpha, CEA, and EMA has been studied in 15 duodenal adenocarcinomas and in eight ampullary adenocarcinomas by avidin-biotin-peroxidase complex indirect immunoperoxidase technique. The positive reaction for p53, c-neu, TGF-alpha, CEA, and EMA in duodenal adenocarcinoma was 20%, 60%, 60%, 73%, and 100%, respectively, and in ampullary adenocarcinoma, 13%, 100%, 50%, 63%, and 100%. Among the duodenal tumors, C-neu and p53 expression was noted more frequently in groups with high histological grades. Patients with c-neu positive duodenal adenocarcinoma had a shorter survival than the patients with c-neu negative duodenal adenocarcinoma (P < 0.01). C-neu product may serve as an unfavorable prognostic indicator in duodenal adenocarcinoma. No statistically significant correlation was found between the expressions of CEA, EMA, p53, and TGF-alpha and patient survival, tumor stage, or histological grade in either duodenal or ampullary adenocarcinomas.
致癌基因、肿瘤抑制基因和生长因子在大多数肿瘤的发生和发展过程中的作用正在被深入研究，但关于十二指肠腺癌或 Vater 壶腹腺癌中癌蛋白或生长因子的表达情况，相关信息却很少。本报告涵盖了 p53、c-neu、转化生长因子-α（TGF-α）、癌胚抗原（CEA）和上皮膜抗原（EMA）在十二指肠腺癌和壶腹腺癌中的表达情况，以及这些表达与肿瘤分期、组织学分级和患者生存率之间的相关性。通过抗生物素蛋白-生物素-过氧化物酶复合物间接免疫过氧化物酶技术，对 15 例十二指肠腺癌和 8 例壶腹腺癌中的 p53、c-neu、TGF-α、CEA 和 EMA 的表达进行了研究。在十二指肠腺癌中，p53、c-neu、TGF-α、CEA 和 EMA 的阳性反应率分别为 20%、60%、60%、73%和 100%；在壶腹腺癌中，分别为 13%、100%、50%、63%和 100%。在十二指肠肿瘤中，c-neu 和 p53 的表达在高组织学分级组中更为常见。c-neu 阳性的十二指肠腺癌患者的生存期比 c-neu 阴性的十二指肠腺癌患者短（P < 0.01）。c-neu 产物可能是十二指肠腺癌预后不良的一个指标。在十二指肠腺癌或壶腹腺癌中，未发现 CEA、EMA、p53 和 TGF-α 的表达与患者生存率、肿瘤分期或组织学分级之间存在统计学上的显著相关性。
