Miyanoshita A, Hara S, Sugiyama M, Asaoka A, Taniai K, Yukuhiro F, Yamakawa M
Laboratory of Biological Defense, National Institute of Sericultural and Entomological Science, Tsukuba, Ibaraki, Japan.
Biochem Biophys Res Commun. 1996 Mar 27;220(3):526-31. doi: 10.1006/bbrc.1996.0438.
A new family member of insect defensin, an antibacterial peptide, has been isolated from larvae of a beetle, Allomyrina dichotoma. The peptide consisted of 43 amino acids and 6 cystein residues were conserved in the same position as that of other insect defensins. The new defensin was found to be inducible by bacterial injection. Analysis of the antibacterial spectrum of A. dichotoma defensin indicated that this peptide showed antibacterial activity against Gram-positive bacteria like Staphylococcus aureus and Bacillus subtilis but not against Gram-negative bacteria like Escherichia coli and Pseudomonas aeruginosa, indicating a typical spectrum of the insect defensin family. In addition, A. dichotoma defensin also exhibited antibacterial activity against methicillin-resistant S. aureus (MRSA) isolated from a patient.
一种新型昆虫防御素（一种抗菌肽）已从独角仙幼虫中分离出来。该肽由43个氨基酸组成，6个半胱氨酸残基在与其他昆虫防御素相同的位置保守存在。发现这种新型防御素可通过注射细菌诱导产生。对独角仙防御素抗菌谱的分析表明，该肽对金黄色葡萄球菌和枯草芽孢杆菌等革兰氏阳性菌具有抗菌活性，但对大肠杆菌和铜绿假单胞菌等革兰氏阴性菌没有抗菌活性，这表明它具有昆虫防御素家族的典型谱型。此外，独角仙防御素对从一名患者分离出的耐甲氧西林金黄色葡萄球菌（MRSA）也表现出抗菌活性。
