系统性红斑狼疮患者免疫球蛋白G组分的直接胚胎毒性
The direct embryotoxicity of immunoglobulin G fractions from patients with systemic lupus erythematosus.
作者信息
Nadler D M, Klein N W, Aramli L A, Chambers B J, Mayes M, Wener M H
机构信息
Department of Animal Science, University of Connecticut, Storrs 06269-4039, USA.
出版信息
Am J Reprod Immunol. 1995 Dec;34(6):349-55. doi: 10.1111/j.1600-0897.1995.tb00963.x.
PROBLEM
To determine if IgG fractions from sera of individuals with systemic lupus erythematosus (SLE) were toxic to cultures of whole rat embryos.
METHODS
Head-fold stage rat embryos (9.5 days of gestation) were cultured on media consisting of 50% rat serum containing IgG fractions isolated from plasmapheresis plasma of six subjects with SLE and six with other autoimmune diseases. Each fraction was tested at 11 mg/ml and those toxic were also tested at 7.5 and 4 mg/ml.
RESULTS
Of the six SLE IgG fractions, four were embryotoxic (embryolethal or teratogenic) while only one of the six non-SLE fractions were embryotoxic.
CONCLUSION
IgG fractions from subjects with SLE can be toxic to cultures of whole rat embryos in the absence of maternal tissues or influence. Such cultures of whole embryos may be useful to identify those antibodies that represent a risk for fetal loss as well as to understand their mechanisms of embryotoxicity.
问题
确定系统性红斑狼疮（SLE）患者血清中的IgG组分对大鼠全胚胎培养物是否具有毒性。
方法
将头褶期大鼠胚胎（妊娠9.5天）培养于含有50%大鼠血清的培养基上，该血清含有从6例SLE患者和6例其他自身免疫性疾病患者的血浆置换血浆中分离出的IgG组分。每个组分在11mg/ml浓度下进行测试，对胚胎有毒性的组分还在7.5mg/ml和4mg/ml浓度下进行测试。
结果
6种SLE IgG组分中，有4种具有胚胎毒性（胚胎致死或致畸），而6种非SLE组分中只有1种具有胚胎毒性。
结论
在没有母体组织或影响的情况下，SLE患者的IgG组分可能对大鼠全胚胎培养物有毒性。这种全胚胎培养物可能有助于识别那些代表胎儿丢失风险的抗体，并了解它们的胚胎毒性机制。
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