Keenan J A, Chen T T, Chadwell N L, Torry D S, Caudle M R
Department of Obstetrics and Gynecology, University of Tennessee Medical Center, Knoxville 37920-6999.
Am J Reprod Immunol. 1994 Oct;32(3):180-3. doi: 10.1111/j.1600-0897.1994.tb01111.x.
The presence of the various cytokines in human peritoneal fluid has been incompletely evaluated. Changes in cytokine levels may be related to activation of peritoneal macrophages, development of endometriosis, and infertility. This study assesses peritoneal fluid levels of interferon gamma (IFN-gamma) and interleukin-6 (IL-6), and peritoneal macrophage production of IL-6, in women with and without endometriosis.
Peritoneal fluid was obtained from 62 women at the time of diagnostic or operative laparoscopic surgery for benign gynecologic disease. Peritoneal macrophages were isolated, cultured for 24 h, and the culture media collected. IFN-gamma and IL-6 levels in peritoneal fluid samples and macrophage conditioned media were determined by commercial ELISA.
IL-6 was significantly higher in the macrophage conditioned media of women with endometriosis as compared with controls. IL-6 levels were fourfold higher in early stage endometriosis (P < 0.05) and eightfold higher in advanced endometriosis. There were no significant differences between groups in the peritoneal fluid levels of IL-6 or IFN-gamma.
Peritoneal macrophage IL-6 secretion is increased in women with endometriosis, and appears to correlate with disease stage. IFN-gamma does not appear to be responsible for the activation of macrophages in women with endometriosis.
人体腹水中多种细胞因子的存在情况尚未得到充分评估。细胞因子水平的变化可能与腹膜巨噬细胞的激活、子宫内膜异位症的发展以及不孕症有关。本研究评估了患有和未患有子宫内膜异位症的女性腹水中干扰素γ（IFN-γ）和白细胞介素-6（IL-6）的水平，以及腹膜巨噬细胞产生IL-6的情况。
在因良性妇科疾病进行诊断性或手术性腹腔镜手术时，从62名女性获取腹水。分离腹膜巨噬细胞，培养24小时，收集培养基。通过商业酶联免疫吸附测定法（ELISA）测定腹水样本和巨噬细胞条件培养基中IFN-γ和IL-6的水平。
与对照组相比，患有子宫内膜异位症的女性巨噬细胞条件培养基中的IL-6显著更高。早期子宫内膜异位症患者的IL-6水平高出四倍（P < 0.05），晚期子宫内膜异位症患者高出八倍。各组间腹水中IL-6或IFN-γ的水平无显著差异。
患有子宫内膜异位症的女性腹膜巨噬细胞IL-6分泌增加，且似乎与疾病阶段相关。IFN-γ似乎与患有子宫内膜异位症女性的巨噬细胞激活无关。
