Heimbürger M, Palmblad J E
Department of Rheumatology, Karolinska Institute at Stockholm Söder Hospital, Sweden.
Clin Exp Immunol. 1996 Mar;103(3):454-60. doi: 10.1111/j.1365-2249.1996.tb08302.x.
We compared the effects of leukotrienes B4, C4 and D4 (LTB4, C4 and D4) in vitro, as well as of histamine and bradykinin, on adhesive interactions between cultured umbilical vein endothelial cells (HUVEC) and polymorphonuclear neutrophils (PMN) and on cytosolic calcium transients, Ca(2+), in vitro. LTB4, but not LTC4 or LTD4 (at 1-100 nM), increased HUVEC adhesiveness for PMN, maximally 2.8 fold; in addition, PMN adhesion was augmented by LTB4 (but not by LTC4 and LTD4) to a plastic surface. Rapid, but smaller increments of HUVEC (but not of PMN) adhesiveness were induced by histamine and bradykinin (at 10 microM). Nonetheless, LTC4 and LTD4 (at 100 nm) induced rapid rises of Ca(2+) in HUVEC, whereas approximately 100-fold higher concentrations were needed of histamine and bradykinin for similar rises. In PMN LTD4 (and LTB4) induced rapid increases of Ca(2+), whereas no significant effect was seen with LTC4, histamine or bradykinin. The Ca(2+) responses to LTC4 and LTD4 were inhibited by the peptidoleukotriene receptor blocker SKF 104,353. Thus, LTB4 and the peptidoleukotrienes display disparate profiles as inducers of adhesion and calcium transients in PMN and HUVEC, indicating discrete differences in the stimulus response coupling for these closely related leukotrienes.
我们比较了白三烯B4、C4和D4（LTB4、C4和D4）以及组胺和缓激肽在体外对培养的脐静脉内皮细胞（HUVEC）与多形核中性粒细胞（PMN）之间黏附相互作用以及对细胞溶质钙瞬变Ca(2+)的影响。LTB4（而非LTC4或LTD4，浓度为1 - 100 nM）可使HUVEC对PMN的黏附性增加，最大增加2.8倍；此外，LTB4（而非LTC4和LTD4）可增强PMN对塑料表面的黏附。组胺和缓激肽（浓度为10 microM）可诱导HUVEC（而非PMN）黏附性快速但较小幅度的增加。尽管如此，LTC4和LTD4（浓度为100 nm）可诱导HUVEC中Ca(2+)快速升高，而组胺和缓激肽需要大约高100倍的浓度才能产生类似升高。在PMN中，LTD4（和LTB4）可诱导Ca(2+)快速增加，而LTC4、组胺或缓激肽则无显著影响。白三烯肽受体阻滞剂SKF 104,353可抑制对LTC4和LTD4的Ca(2+)反应。因此，LTB4和肽白三烯在PMN和HUVEC中作为黏附诱导剂和钙瞬变诱导剂表现出不同的特征，表明这些密切相关的白三烯在刺激反应偶联方面存在明显差异。
