Warembourg M
Ann Endocrinol (Paris). 1977;38(1):41-54.
The central nervous system and pituitary of various mammals were examined by radioautography after injection of different tritiated steroid hormones. After injection of 3H estradiol into ovariectomized mice, radioautograms revealed a significant labelling in cells of the amygdala, the bed nucleus of the stria terminalis, the nucleus preopticus medialis, the nuclei arcuatus and ventro-medialis. The radioactive material was associated with the cell nuclei. Certain anterior lobe cells retained the hormone. After injection of 3H testosterone into castrated rats, the central nervous system and the anterior pituitary contained labelled cells. In the hypothalamus, the distribution pattern of androgen-neurons appears to be similar from the estrogen-neuron areas, although some differences in the number of cells and the intensity of labelling seen to exist. After injection of 3H progesterone into castrated estrogen-primed guinea-pigs, labelled neurons have been observed in the regions of nucleus arcuatus and nucleus preopticus suprachiasmaticus. However, the presence of progesterone receptors in the hypothalamus has not been still clearly demonstrated. After injection of 3H corticosterone into adrenalectomized male rats, radioactivity was found to be selectively concentrated in neurons of septum, hippocampal complex (precommissural hippocampus, cornu Ammonis, gyrus dentatus, subiculum) indusium griseum, amygdala and in certain areas of the cortex. Most of the silver grains were localized in the nuclei of labelled cells. On the other hand, after injection of 3H dexamethasone radioactivity concentration was high in the medial basal hypothalamus, in the anterior pituitary and in the pineal gland. Differences appear to exist in the topographic distribution of dexamethasone and corticosterone-concentrating cells.
给不同的哺乳动物注射不同的氚标记类固醇激素后，用放射自显影法检查了它们的中枢神经系统和垂体。给去卵巢的小鼠注射³H雌二醇后，放射自显影片显示杏仁核、终纹床核、内侧视前核、弓状核和腹内侧核的细胞有明显的标记。放射性物质与细胞核有关。某些前叶细胞保留了这种激素。给去势大鼠注射³H睾酮后，中枢神经系统和垂体前叶含有标记细胞。在下丘脑，雄激素神经元的分布模式似乎与雌激素神经元区域相似，尽管在细胞数量和标记强度上存在一些差异。给经雌激素预处理的去势豚鼠注射³H孕酮后，在弓状核和视交叉上核区域观察到了标记神经元。然而，下丘脑孕酮受体的存在仍未得到明确证实。给肾上腺切除的雄性大鼠注射³H皮质酮后，发现放射性物质选择性地集中在隔区、海马复合体（连合前海马、海马、齿状回、海马下脚）、灰被、杏仁核和皮质的某些区域的神经元中。大多数银粒位于标记细胞的细胞核中。另一方面，注射³H地塞米松后，内侧基底下丘脑、垂体前叶和松果体中的放射性物质浓度较高。地塞米松和皮质酮浓缩细胞的地形分布似乎存在差异。
