λ阻遏物在体内结合的DNA序列决定因素。
DNA sequence determinants of lambda repressor binding in vivo.
作者信息
Benson N, Sugiono P, Youderian P
机构信息
Department of Biological Sciences, University of Southern California, Los Angeles, 90089-1481, USA.
出版信息
Genetics. 1988 Jan;118(1):21-9. doi: 10.1093/genetics/118.1.21.
Abstract
The critical operator determinants for lambda repressor recognition have been defined by analyzing the binding of wild-type repressor to a set of mutant operators in vivo. Base pair substitutions at six positions within the lambda operator half-site impair binding severely, and define these base pairs as critical for operator function. One mutant operator binds repressor better than the consensus operator, and is a superoperator. The model proposed by M. Lewis in 1983 for the binding of lambda repressor to its operator accurately predicts the observed operator requirements for binding in vivo, with several minor exceptions. The order of affinities of the six natural lambda operators has also been determined.
摘要
通过分析野生型阻遏物在体内与一组突变操纵基因的结合情况，确定了λ阻遏物识别的关键操纵基因决定因素。λ操纵基因半位点内六个位置的碱基对替换会严重损害结合，并将这些碱基对定义为操纵基因功能的关键因素。一个突变操纵基因比共有操纵基因与阻遏物的结合更好，是一个超级操纵基因。M. 刘易斯在1983年提出的关于λ阻遏物与其操纵基因结合的模型准确地预测了在体内观察到的结合所需的操纵基因条件，但有几个小的例外。还确定了六个天然λ操纵基因的亲和力顺序。
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