Koshino T, Arai Y, Miyamoto Y, Sano Y, Itami M, Teshima S, Hirai K, Takaishi T, Ito K, Morita Y
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Japan.
J Asthma. 1996;33(2):89-95. doi: 10.3109/02770909609054536.
We investigated the role of basophils and mast cells in the pathogenesis of bronchial asthma. Eight asthmatics (6 atopic, 2 nonatopic) and 6 control subjects were enrolled in this study. Bronchial responsiveness to acetylcholine (PC20ACh) was measured in asthmatics and endobronchial biopsy from right upper lobe bronchus was performed on the same day. Basophils and mast cells in the airways were identified by immunohistochemistry using a monoclonal antibody against tryptase and anti-IgE. The number of basophils of asthmatics was 52.2 +/- 12.5/mm(2). In contrast, no basophils were found in the airways of control subjects. There was a significant increase of number of mast cells in the asthma group compared to the control group (168.6 +/- 32.6 vs. 22.3 +/- 6.1, p<0.01). There was an inverse correlation between airway basophil and mast cell numbers and PC20ACh (r=-0.82, r=0.72, p<0.05). These findings suggest a possible role for basophils and mast cells in the pathophysiology of asthma.
我们研究了嗜碱性粒细胞和肥大细胞在支气管哮喘发病机制中的作用。本研究纳入了8名哮喘患者（6名特应性患者，2名非特应性患者）和6名对照受试者。在哮喘患者中测量了对乙酰胆碱的支气管反应性（PC20ACh），并于同日对右上叶支气管进行了支气管内活检。使用抗胰蛋白酶单克隆抗体和抗IgE通过免疫组织化学鉴定气道中的嗜碱性粒细胞和肥大细胞。哮喘患者的嗜碱性粒细胞数量为52.2±12.5/mm(2)。相比之下，在对照受试者的气道中未发现嗜碱性粒细胞。与对照组相比，哮喘组的肥大细胞数量显著增加（168.6±32.6对22.3±6.1，p<0.01）。气道嗜碱性粒细胞和肥大细胞数量与PC20ACh之间存在负相关（r=-0.82，r=0.72，p<0.05）。这些发现提示嗜碱性粒细胞和肥大细胞在哮喘病理生理学中可能发挥作用。
