Rolph M S, Ramshaw I A, Rockett K A, Ruby J, Cowden W B
Division of Cell Biology, John Curtin School of Medical Research, Canberra, Australia.
Virology. 1996 Mar 15;217(2):470-7. doi: 10.1006/viro.1996.0141.
Recent reports have highlighted a potential antiviral activity for nitric oxide (NO). The purpose of this study was to investigate the production of NO in mice during vaccinia virus (VV) or herpes simplex virus type 1 infection, and to assess the role of NO in clearance of VV. Reactive nitrogen intermediates (RNI; NO and its stable oxidation products, nitrite and nitrate) were significantly elevated in the plasma of mice infected with these viruses. Furthermore, spleen cells from virus-infected mice produced elevated RNI levels following stimulation in vitro with LPS. NO production during VV infection was critically dependent on the cytokines tumor necrosis factor and interferon-gamma, and on the presence of both CD4+ and CD8+ T lymphocytes. Treatment of VV-infected mice with the nitric oxide synthase inhibitor N(G)-methyl-L-arginine did not alter the course of infection, suggesting that NO may not be essential for the clearance of this virus.
近期报告强调了一氧化氮（NO）的潜在抗病毒活性。本研究的目的是调查小鼠在感染痘苗病毒（VV）或1型单纯疱疹病毒期间NO的产生情况，并评估NO在清除VV中的作用。感染这些病毒的小鼠血浆中活性氮中间体（RNI；NO及其稳定氧化产物亚硝酸盐和硝酸盐）显著升高。此外，病毒感染小鼠的脾细胞在体外经脂多糖刺激后产生的RNI水平升高。VV感染期间NO的产生严重依赖于细胞因子肿瘤坏死因子和干扰素-γ，以及CD4+和CD8+ T淋巴细胞的存在。用一氧化氮合酶抑制剂N(G)-甲基-L-精氨酸治疗VV感染的小鼠并未改变感染进程，这表明NO可能对于清除该病毒并非必不可少。
