一种用于测量肌酐的新酶法，涉及一种新型的ATP依赖性酶，N-甲基乙内酰脲酰胺水解酶。

A new enzymatic method for the measurement of creatinine involving a novel ATP-dependent enzyme, N-methylhydantoin amidohydrolase.

作者信息

Ogawa J, Nirdnoy W, Tabata M, Yamada H, Shimizu S

机构信息

Department of Agricultural Chemistry, Kyoto University, Japan.

出版信息

Biosci Biotechnol Biochem. 1995 Dec;59(12):2292-4. doi: 10.1271/bbb.59.2292.

DOI:10.1271/bbb.59.2292

PMID:8611752

Abstract

A new enzymatic method for the measurement of serum and urine creatinine is described. The method is based on a novel microbial creatinine degradation pathway via N-methylhydantoin [Shimizu et al., Clin. Chim. Acta, 185, 241-252 (1989)]. By using two novel enzymes, N-methylhydantoin amidohydrolase and N-carbamoylsarcosine amidohydrolase, as key enzymes, coupled with a colorimetric procedure for hydrogen peroxide detection, the creatinine level can be measured. The results obtained for human serum and urine show good correlation with those obtained by a standard chemical method based on the Jaffe reaction. The new method is simple and specific, and shows excellent sensitivity and reliability.

摘要

描述了一种用于测量血清和尿液肌酐的新酶法。该方法基于一种通过N-甲基乙内酰脲的新型微生物肌酐降解途径[Shimizu等人，《临床化学学报》，185，241 - 252(1989)]。通过使用两种新型酶，即N-甲基乙内酰脲酰胺水解酶和N-氨甲酰基肌氨酸酰胺水解酶作为关键酶，并结合用于检测过氧化氢的比色法，可测量肌酐水平。人血清和尿液的检测结果与基于Jaffe反应的标准化学方法所获得的结果具有良好的相关性。新方法简单且特异，具有出色的灵敏度和可靠性。