用高效液相色谱法分析肝脏和脑微粒体体外对丙咪嗪的代谢。

High-performance liquid chromatographic method for the analysis of imipramine metabolism in vitro by liver and brain microsomes.

作者信息

Sequeira D J, Strobel H W

机构信息

Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77225, USA.

出版信息

J Chromatogr B Biomed Appl. 1995 Nov 17;673(2):251-8. doi: 10.1016/0378-4347(95)00274-x.

DOI:10.1016/0378-4347(95)00274-x

PMID:8611959

Abstract

A new sensitive method for resolution and quantitation metabolites of in vitro imipramine metabolism has been developed for use in liver and brain microsomes. Separation of metabolites was done using a Supelcosil PCN column with a mobile phase of acetonitrile-methanol-potassium phosphate dibasic (40:35:25, v/v/v), pH 7. Resolution is achieved for 2- and 10-hydroxyimipramine, and desipramine. Varying levels of these metabolites formed during in vitro incubations of rat liver and brain microsomes following treatments.

摘要

已开发出一种用于体外丙咪嗪代谢产物分离和定量的新灵敏方法，用于肝和脑微粒体。代谢产物的分离使用Supelcosil PCN柱，流动相为乙腈-甲醇-磷酸氢二钾（40:35:25，v/v/v），pH值为7。可实现对2-羟基丙咪嗪、10-羟基丙咪嗪和地昔帕明的分离。在大鼠肝和脑微粒体经处理后的体外孵育过程中会形成不同水平的这些代谢产物。