一种用于测定房水中氧氟沙星浓度的超高效液相色谱-质谱法。
A UPLC-MS Method for the Determination of Ofloxacin Concentrations in Aqueous Humor.
作者信息
Plotas Panagiotis, Anastasopoulos Charalampos, Makri Olga E, Leotsinidis Michalis, Georgakopoulos Constantine D
机构信息
Department of Ophthalmology, Medical School, University of Patras, Patras, Greece. ; Laboratory of Public Health, School of Medicine, University of Patras, Patras, Greece.
Department of Chemistry, University of Patras, Patras, Greece.
出版信息
Anal Chem Insights. 2014 May 5;9:27-32. doi: 10.4137/ACI.S13857. eCollection 2014.
A rapid, simple, and specific method based on ultra performance liquid chromatography (UPLC) with mass spectrometry detection has been developed for quantitative analysis of ofloxacin in human aqueous humor using tobramycin as internal standard (IS). Chromatographic separation was achieved on a Waters Acquity UPLC BEH C18 Shield column (150 × 2.1 mm, 1.7 μm) eluted with 95:5 water: acetonitrile (v/v) containing 0.1% formic acid and a flow rate of 0.3 mL/minute. The total analysis time was three minutes with ofloxacin eluting at 1.67 ± 0.03 minutes. The linearity of the method ranged from 0.1 to 8 μg/mL with r2 = 0.998. The method was validated according to FDA guidelines with respect to linearity, accuracy, precision, specificity, and stability. The limits of detection and quantification were 0.03 and 0.10 μg/mL, respectively. The developed method was successfully applied to the analysis of samples that have been obtained from patients.
已开发出一种基于超高效液相色谱（UPLC）与质谱检测联用的快速、简单且特异的方法，以妥布霉素作为内标（IS），用于定量分析人房水中的氧氟沙星。在Waters Acquity UPLC BEH C18 Shield柱（150×2.1 mm，1.7μm）上进行色谱分离，以含0.1%甲酸的95:5水:乙腈（v/v）为流动相，流速为0.3 mL/分钟。总分析时间为3分钟，氧氟沙星在1.67±0.03分钟时洗脱。该方法的线性范围为0.1至8μg/mL，r2 = 0.998。根据美国食品药品监督管理局（FDA）指南，对该方法的线性、准确度、精密度、特异性和稳定性进行了验证。检测限和定量限分别为0.03和0.10μg/mL。所开发的方法成功应用于从患者获取的样本分析。
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