Endo T, Fujiwara T, Ikehara Y, Kobata A
Department of Biochemistry, Institute of Medical Science, University of Tokyo, Japan.
Eur J Biochem. 1996 Mar 1;236(2):579-90. doi: 10.1111/j.1432-1033.1996.t01-1-00579.x.
The N-linked sugar chains of alkaline phosphatases, purified from rat AH-130 hepatoma and from normal rat liver, were released quantitatively as oligosaccharides by hydrazinolysis and were labeled by reduction with NaB3H4. A comparative study of their structures revealed that following structural differences are induced by hepatocyte carcinogenesis: complex-type tetraantennary sugar chains and hybrid-type sugar chains appear; outer-chain moieties of the sugar chains of the hepatoma enzyme contain exclusively the Gal(Beta 1-4)GlcNAc groups (type 2 chains) but those of the normal enzyme contain other Gal(Beta 1-)GlcNAc groups and type 2 chains; and novel fucosylated high-mannose-type sugar chains are found in the oligosaccharides of the hepatoma enzyme.
从大鼠AH - 130肝癌组织和正常大鼠肝脏中纯化得到的碱性磷酸酶，其N - 连接糖链经肼解定量释放为寡糖，并用NaB3H4还原进行标记。对它们结构的比较研究表明，肝细胞癌变会引发以下结构差异：出现复合型四天线糖链和杂合型糖链；肝癌酶糖链的外链部分仅含有Gal(β1 - 4)GlcNAc基团（2型链），而正常酶的外链部分含有其他Gal(β1 - )GlcNAc基团和2型链；并且在肝癌酶的寡糖中发现了新型岩藻糖基化的高甘露糖型糖链。
