Pizette S, Coulier F, Birnbaum D, DeLapeyrière O
Laboratoire d'Oncologie Moleculaire, U. 119 INSERM, Marseille, France.
Exp Cell Res. 1996 Apr 10;224(1):143-51. doi: 10.1006/excr.1996.0122.
Fgf6 is the only known member of the FGF family whose expression is restricted to the muscle cell lineage during development, suggesting it may have a role in myogenesis. Muscle satellite cells but not C2 myoblast cells were found to express Fgf6. We have used purified recombinant FGF6 protein to explore the effect of this factor on C2 cells in culture. FGF6 stimulated the proliferation of C2 myoblasts and, in combination with heparin, induced their morphological transformation. FGF6, added at 5 ng/ml and in the presence of heparin, increased the expression of a subset of muscle cell differentiation markers. In contrast, at 25 ng/ml, it down-regulated the expression of myogenic markers and myogenic transcription factors examined and delayed differentiation into myotubes of C2 cells. It also up-regulated the expression of FgfR1 and had an opposite effect on FgfR4. These results suggest that intramuscular FGF6 concentrations could influence the proliferation and differentiation processes taking place during development.
Fgf6是成纤维细胞生长因子（FGF）家族中唯一已知的成员，其表达在发育过程中仅限于肌肉细胞谱系，这表明它可能在肌生成中发挥作用。研究发现，肌肉卫星细胞而非C2成肌细胞表达Fgf6。我们使用纯化的重组FGF6蛋白来探索该因子对培养的C2细胞的影响。FGF6刺激C2成肌细胞的增殖，并与肝素联合诱导其形态转变。在5 ng/ml且存在肝素的情况下添加FGF6，可增加一部分肌肉细胞分化标志物的表达。相比之下，在25 ng/ml时，它下调了所检测的成肌标志物和成肌转录因子的表达，并延迟了C2细胞分化为肌管。它还上调了FgfR1的表达，而对FgfR4则有相反的作用。这些结果表明，肌肉内FGF6的浓度可能会影响发育过程中发生的增殖和分化过程。
