Richardson M, Hadcock S J, Hatton B D, Winocour P D, Hatton M W
Department of Pathology, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada.
Exp Mol Pathol. 1995 Jun;62(3):145-59. doi: 10.1006/exmp.1995.1016.
Proteoglycans and glycosaminoglycans in the aortic intima of diabetic rabbits and age-matched controls were examined at 2 weeks and 3, 6, and 12 months after alloxan (or saline) treatment. Measurements were made by morphometric analysis of ruthenium red-stained large proteoglycan granules (LPG) in electron micrographs and by analysis of 35S-labeled glycosaminoglycans, extracted and purified from the intima-media of aortas of rabbits which had been injected with 35S-sulfate 18 hr before exsanguination. There was a progressive increase in the area of the aortic intima with time which was greater in diabetic than in control rabbits. The concentration of proteoglycan (LPG/microns 2) and the concentration of the 35S-glycosaminoglycans in diabetic intima-media were similar to respective values of control intima-media throughout the 12 months. However, the specific radioactivity of the [35S]glycosaminoglycan pool from intima-media of diabetic rabbits was significantly less than that from controls (P < 0.001) at 6 and 12 months. In addition, the staining intensity of LPG of the diabetic compared to control extracellular matrix was decreased at these times. The profile and electrophoretic mobility of the glycosaminoglycan types were similar in diabetic and control intima-media. We conclude that the onset of diabetes in the rabbit has altered the metabolic turnover but not the concentration, sulfate content or profile of aortic intima-media proteoglycan and glycosaminoglycans.
在给予四氧嘧啶（或生理盐水）治疗后的2周以及3、6和12个月，对糖尿病兔和年龄匹配的对照兔主动脉内膜中的蛋白聚糖和糖胺聚糖进行了检测。通过对电子显微镜下钌红染色的大蛋白聚糖颗粒（LPG）进行形态计量分析，以及对从放血前18小时注射35S-硫酸盐的兔主动脉内膜-中膜中提取和纯化的35S标记糖胺聚糖进行分析来进行测量。随着时间的推移，主动脉内膜面积逐渐增加，糖尿病兔的增加幅度大于对照兔。在整个12个月期间，糖尿病内膜-中膜中蛋白聚糖的浓度（LPG/μm2）和35S-糖胺聚糖的浓度与对照内膜-中膜的相应值相似。然而，在6个月和12个月时，糖尿病兔内膜-中膜中[35S]糖胺聚糖池的比放射性显著低于对照兔（P<0.001）。此外，在这些时间点，与对照细胞外基质相比，糖尿病LPG的染色强度降低。糖尿病和对照内膜-中膜中糖胺聚糖类型的分布和电泳迁移率相似。我们得出结论，兔糖尿病的发生改变了主动脉内膜-中膜蛋白聚糖和糖胺聚糖的代谢周转，但未改变其浓度、硫酸盐含量或分布。
