Suzuki G, Ohta H, Kato T, Igarashi T, Sakai F, Shibata D, Takano A, Masuda T, Shioi Y, Takamiya K
Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Yokohama, Japan.
FEBS Lett. 1996 Mar 25;383(1-2):83-6. doi: 10.1016/0014-5793(96)00229-3.
We isolated a cDNA encoding a novel cytochrome P450 (CYP93A1) from soybean suspension-cultured cells that had been treated with methyl jasmonate (MeJA). The amino acid sequence of the gene product had 30-40% identity with those of other plant P450s. The protein contained the heme-binding domain which is highly conserved among plant P450s. Transcription of the cytochrome P450 gene in soybean cells was induced by 30 microM MeJA even in the presence of cycloheximide, and reached maximum level 6 h after MeJA treatment. This is the first report of a plant cytochrome P450 gene whose transcription is induced by MeJA even without protein synthesis.
我们从经茉莉酸甲酯（MeJA）处理的大豆悬浮培养细胞中分离出一个编码新型细胞色素P450（CYP93A1）的cDNA。该基因产物的氨基酸序列与其他植物P450的氨基酸序列具有30%-40%的同一性。该蛋白质含有在植物P450中高度保守的血红素结合结构域。即使在存在环己酰亚胺的情况下，大豆细胞中细胞色素P450基因的转录也会被30微摩尔的MeJA诱导，并在MeJA处理后6小时达到最高水平。这是关于一种植物细胞色素P450基因的首次报道，其转录即使在没有蛋白质合成的情况下也能被MeJA诱导。
