Artinger K B, Fraser S, Bronner-Fraser M
Developmental Biology Center, University of California, Irvine 92717, USA.
Dev Biol. 1995 Dec;172(2):591-601. doi: 10.1006/dbio.1995.8038.
To challenge the developmental potential of dorsal neural tube cells and test whether single neuroepithelial cells can give rise to the full range of neural tube derivatives, we grated a notochord lateral to the closing neural folds. This results in juxtaposition of dorsal and ventral cell types, by inducing floor plate cells and motor neurons dorsally. Clonal analysis with the vital dye lysinated rhodamine dextran showed that both "dorsal" and "ventral" neural tube derivatives can arise from a single precursor. Cells as diverse as sensory ganglion cells, presumptive pigment cells, roof plate cells, motor neurons, and floor plate cells were observed in the same clone. The presence of such diversity within single clones indicates that the responses to dorsal and ventral signals are not mutually exclusive; even in the early neural tube, neuroepithelial cells are not restricted to form only dorsal or ventral neural tube derivatives.
为了挑战背侧神经管细胞的发育潜能，并测试单个神经上皮细胞是否能产生所有类型的神经管衍生物，我们在正在闭合的神经褶侧面移植了一根脊索。这通过在背侧诱导底板细胞和运动神经元，导致背侧和腹侧细胞类型并列存在。用活性染料赖氨酸化罗丹明葡聚糖进行的克隆分析表明，“背侧”和“腹侧”神经管衍生物都可源自单个前体细胞。在同一个克隆中观察到了多种不同类型的细胞，如感觉神经节细胞、假定的色素细胞、顶板细胞、运动神经元和底板细胞。单个克隆中存在如此多样的细胞类型，表明对背侧和腹侧信号的反应并非相互排斥；即使在早期神经管中，神经上皮细胞也不限于仅形成背侧或腹侧神经管衍生物。
