Lohse A W, Knolle P A, Bilo K, Uhrig A, Waldmann C, Ibe M, Schmitt E, Gerken G, Meyer Zum Büschenfelde K H
Department of Medicine, Johannes Gutenberg University, Mainz, Germany.
Gastroenterology. 1996 Apr;110(4):1175-81. doi: 10.1053/gast.1996.v110.pm8613007.
BACKGROUND & AIMS: Inflammatory liver disease as well as rejection of liver allografts are thought to be mediated by resident antigen-presenting cells in the liver. At the same time, in vivo antigen presentation in the liver appears to be a more tolerogenic than systemic antigen challenge. The aim of this study was to show and characterize the antigen-presenting capability of sinusoidal endothelial cells and Kupffer cells.
Purified murine sinusoidal endothelial cells and Kupffer cells were studied for their ability to serve as accessory cells and antigen-presenting cells by proliferation assays. They were also studied for their expression of interleukin 1 and the B7 costimulatory molecules by Northern blotting, polymerase chain reaction, and flow cytometry.
Both cell types expressed interleukin 1 messenger RNA and could serve equally well as accessory and antigen-presenting cells. B7-2 messenger RNA and surface expression on sinusoidal endothelial cells and on Kupffer cells was shown. Antibodies to the B7 molecules inhibited antigen presentation. Addition of interleukin 10 as a regulatory cytokine secreted by Kupffer cells was suppressive.
Sinusoidal endothelial cells carry functional B7-2 molecules and can serve as effective antigen-presenting cells. However, antigen presentation by sinusoidal endothelial cells may be locally down-regulated by interleukin 10.
炎症性肝病以及肝移植排斥反应被认为是由肝脏中的驻留抗原呈递细胞介导的。同时，肝脏中的体内抗原呈递似乎比全身性抗原刺激更具耐受性。本研究的目的是展示并表征肝血窦内皮细胞和库普弗细胞的抗原呈递能力。
通过增殖试验研究纯化的小鼠肝血窦内皮细胞和库普弗细胞作为辅助细胞和抗原呈递细胞的能力。还通过Northern印迹、聚合酶链反应和流式细胞术研究它们白细胞介素1和B7共刺激分子的表达。
两种细胞类型均表达白细胞介素1信使核糖核酸，并且作为辅助细胞和抗原呈递细胞的效果相同。显示了肝血窦内皮细胞和库普弗细胞上B7-2信使核糖核酸和表面表达。针对B7分子的抗体抑制抗原呈递。添加作为库普弗细胞分泌的调节性细胞因子的白细胞介素10具有抑制作用。
肝血窦内皮细胞携带功能性B7-2分子，可作为有效的抗原呈递细胞。然而，肝血窦内皮细胞的抗原呈递可能会被白细胞介素10局部下调。
