Zhang T, Stanley S L
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Infect Immun. 1996 May;64(5):1526-31. doi: 10.1128/iai.64.5.1526-1531.1996.
Attenuated salmonellae represent attractive candidates for the delivery of foreign antigens by oral vaccination. In this report, we describe the high-level expression of a recombinant fusion protein containing the serine-rich Entamoeba histolytica protein (SREHP), a protective antigen derived from virulent amebae, and a bacterially derived maltose-binding protein (MBP) in an attenuated strain of Salmonella typhimurium. Mice and gerbils immunized with S. typhimurium expressing SREHP-MBP produced mucosal immunoglobulin A antiamebic antibodies and serum immunoglobulin G antiamebic antibodies. Gerbils vaccinated with S typhimurium SREHP-MBP were protected against amebic liver abscess, the most common extraintestinal complication of amebiasis. Our findings indicate that the induction of mucosal and immune responses to the amebic SREHP antigen is dependent on the level of SREHP-MBP expression in S. typhimurium and establish that oral vaccination with SREHP can produce protective immunity to invasive amebiasis.
减毒沙门氏菌是通过口服疫苗递送外源抗原的有吸引力的候选者。在本报告中，我们描述了一种重组融合蛋白在鼠伤寒沙门氏菌减毒株中的高水平表达，该重组融合蛋白包含富含丝氨酸的溶组织内阿米巴蛋白（SREHP），一种源自有毒力阿米巴的保护性抗原，以及细菌来源的麦芽糖结合蛋白（MBP）。用表达SREHP-MBP的鼠伤寒沙门氏菌免疫的小鼠和沙鼠产生了粘膜免疫球蛋白A抗阿米巴抗体和血清免疫球蛋白G抗阿米巴抗体。用鼠伤寒沙门氏菌SREHP-MBP接种的沙鼠对阿米巴肝脓肿具有抵抗力，阿米巴肝脓肿是阿米巴病最常见的肠外并发症。我们的研究结果表明，对阿米巴SREHP抗原的粘膜和免疫反应的诱导取决于鼠伤寒沙门氏菌中SREHP-MBP的表达水平，并证实用SREHP进行口服疫苗接种可产生针对侵袭性阿米巴病的保护性免疫。
