Smith Q T, Harriman L, Au G S, Stoltenberg J L, Osborn J B, Aeppli D M, Fischer G
Clinical Research Center for Periodontal Diseases, University of Minnesota, Minneapolis, USA.
J Clin Periodontol. 1995 Dec;22(12):935-41. doi: 10.1111/j.1600-051x.1995.tb01798.x.
Neutrophil elastase (NE) was measured in crevicular fluid (GCF) collected from 3 subject groups. GCF was harvested at a single visit of subjects with periodontal health (n = 21) and with periodontitis (n = 28). Samples were obtained from 132 middle-aged, middle-class health conscious patients of a health maintenance organization (HMO) at baseline and 1 year later. GCF NE was higher in periodontitis than in health. Mean GCF NE of HMO subjects was much closer to health than to periodontitis. Few members of the HMO population had enzyme levels typical of periodontitis. Subjects and sites of the HMO population were segregated into 3 categories based on enzyme levels of the healthy and periodontitis subjects. Most HMO subjects and sites were in the activity category corresponding to healthy subjects. Only a small portion were in the activity category common in periodontitis. Enzyme levels in the highest activity category at both samplings were infrequent. High enzyme levels in the HMO population were not associated with attachment loss. Thus, assay of GCF NE provided little evidence of disease in a middle-aged, middle-class health conscious general population. This finding confirms an analysis of epidemiological surveys which concluded that a population such as studied here would not benefit from periodontal diagnostic testing.
在从3个受试者组收集的龈沟液（GCF）中检测中性粒细胞弹性蛋白酶（NE）。在牙周健康受试者（n = 21）和牙周炎受试者（n = 28）单次就诊时采集GCF。从一个健康维护组织（HMO）的132名具有健康意识的中年中产阶级患者基线期和1年后获取样本。牙周炎患者的GCF NE高于健康者。HMO受试者的平均GCF NE更接近健康者而非牙周炎患者。HMO人群中很少有成员具有典型的牙周炎酶水平。根据健康受试者和牙周炎受试者的酶水平，将HMO人群的受试者和部位分为3类。大多数HMO受试者和部位属于与健康受试者相对应的活动类别。只有一小部分属于牙周炎中常见的活动类别。两次采样时最高活动类别的酶水平都不常见。HMO人群中的高酶水平与附着丧失无关。因此，检测GCF NE几乎没有为中年中产阶级健康意识普遍人群中的疾病提供证据。这一发现证实了一项流行病学调查分析的结论，即像这里所研究的人群不会从牙周诊断测试中受益。
