Gregory C J, Eaves A C
Blood. 1977 Jun;49(6):855-64.
A systematic study has been undertaken to analyze the spectrum of erythropoietic colonies obtained in cultures of human marrow cells plated in methyl cellulose. Colonies were identified as erythropoietic on the basis of the appearance in them of hemoglobin-containing erythroblasts. As found previously in mouse marrow cultures, three sequentially appearing types of colonies which differed in their ultimate cluster content could be readily distinguished. Small erythroid colonies containing 1-2 clusters reached a peak after 7-8 days; small bursts containing 3-8 clusters reached a peak after 10-12 days; and large bursts containing greater than 16 clusters reached a peak after 17-20 days. The previously reported enhancing effect of human leukocyte conditioned medium on burst formation seen in cultures of human nonadherent cells was found to be due largely to an effect on the formation of the largest, late appearing type of burst. By analogy with the mouse, the progenitors of such bursts would represent a primitive cell type which has a close relationship with pluripotent stem cells, as well as a second and independent close relationship to the progenitors of granulopoietic colonies.
已开展一项系统性研究，以分析接种于甲基纤维素中的人骨髓细胞培养物中获得的红细胞生成集落谱系。根据含血红蛋白的成红细胞在集落中的出现情况，将集落鉴定为红细胞生成集落。正如先前在小鼠骨髓培养物中所发现的，可轻松区分出三种依次出现的集落类型，它们最终的集簇含量有所不同。含有1 - 2个集簇的小红系集落在7 - 8天后达到峰值；含有3 - 8个集簇的小爆式集落在10 - 12天后达到峰值；含有超过16个集簇的大爆式集落在17 - 20天后达到峰值。先前报道的人白细胞条件培养基对人非贴壁细胞培养物中爆式集落形成的增强作用，在很大程度上被发现是由于对最大的、后期出现的爆式集落形成的影响。与小鼠类似，此类爆式集落的祖细胞将代表一种原始细胞类型，它与多能干细胞有密切关系，并且与粒细胞生成集落的祖细胞还有第二种独立的密切关系。
