Israel Z R, Marx P A
Department of Pathology, New York University Medical Center, NY, USA.
J Med Primatol. 1995 Feb;24(2):53-60. doi: 10.1111/j.1600-0684.1995.tb00146.x.
To identify mucosal immunity in HIV-infected chimpanzees, IgG, IgA, and IgM from plasma, saliva, rectal swabs, vaginal washes, semen, and urethral washes were tested from four male and three female HIV-1IIIB infected chimpanzees. The level of HIV infections in the seven chimpanzees were classified as high, intermediate and low depending on the number of HIV-1 infected cells per 10(7) peripheral blood mononuclear cells (PBMC). One male chimpanzee had a relatively high viral load, two males and two females had moderate viral loads and one male and one female had low levels of infection. All seven animals had plasma antibody. The principal finding was that nonclassical mucosal antibodies of the IgG isotype were the predominant antibody in the saliva, rectal swabs, vaginal washes, semen, and urethral washes of infected animals. All plasma and mucosal samples were negative for IgM antibodies. The results show that HIV-1 specific IgG responses and not sIgA predominate at mucosal surfaces of HIV-1IIIB infected chimpanzees. A trend was observed in which high viral loads correlated with high plasma IgG, IgA and sIgA titers. An overall correlation between relatively high virus loads and high amounts of mucosal IgG was also found.
为了鉴定感染HIV的黑猩猩的黏膜免疫情况，对4只雄性和3只雌性感染HIV-1IIIB的黑猩猩的血浆、唾液、直肠拭子、阴道灌洗液、精液和尿道灌洗液中的IgG、IgA和IgM进行了检测。根据每10⁷外周血单个核细胞（PBMC）中HIV-1感染细胞的数量，将这7只黑猩猩的HIV感染水平分为高、中、低三类。一只雄性黑猩猩病毒载量相对较高，两只雄性和两只雌性病毒载量中等，一只雄性和一只雌性感染水平较低。所有7只动物血浆中均有抗体。主要发现是，在受感染动物的唾液、直肠拭子、阴道灌洗液、精液和尿道灌洗液中，IgG同种型的非经典黏膜抗体是主要抗体。所有血浆和黏膜样本的IgM抗体均为阴性。结果表明，在感染HIV-1IIIB的黑猩猩的黏膜表面，HIV-1特异性IgG反应而非分泌型IgA占主导。观察到一种趋势，即高病毒载量与高血浆IgG、IgA和分泌型IgA滴度相关。还发现相对较高的病毒载量与大量黏膜IgG之间存在总体相关性。
