通过粒子介导将重组表达载体递送至兔皮肤可诱导产生高滴度的多克隆抗血清（并且无需纯化蛋白质免疫原）。
Particle-mediated delivery of recombinant expression vectors to rabbit skin induces high-titered polyclonal antisera (and circumvents purification of a protein immunogen).
作者信息
Sundaram P, Xiao W, Brandsma J L
机构信息
Section of Comparative Medicine, Yale University School of Medicine, New Haven, CT 06520-8016, USA.
出版信息
Nucleic Acids Res. 1996 Apr 1;24(7):1375-7. doi: 10.1093/nar/24.7.1375.
Abstract
Polyclonal antibodies were generated in rabbits by delivery to skin of gold particles coated with mammalian expression vectors encoding a cytoplasmic (beta-galactosidase) or a nuclear (L1 capsid of cottontail rabbit papillomavirus) protein. One primary and one booster immunization of 30 micrograms DNA per rabbit yielded specific antisera with titers from 1:24 000 to 1:120 000 in each of eight rabbits, as detected by ELISA and Western blot analysis. Genetic immunization requires relatively small amounts of DNA, eliminates the need to purify the protein immunogen, and does not require irritating adjuvants.
摘要
通过将包被有编码细胞质蛋白（β-半乳糖苷酶）或核蛋白（棉尾兔乳头瘤病毒L1衣壳蛋白）的哺乳动物表达载体的金颗粒注入兔皮肤，产生多克隆抗体。每只兔子进行一次30微克DNA的初次免疫和一次加强免疫，通过ELISA和蛋白质印迹分析检测，八只兔子中的每只都产生了滴度为1:24 000至1:120 000的特异性抗血清。基因免疫所需的DNA量相对较少，无需纯化蛋白质免疫原，也不需要刺激性佐剂。
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