Augustine P C
USDA, Parasite Biology and Epidemiology Laboratory, Beltsville, Maryland 20705-2350, USA.
Poult Sci. 1995 Nov;74(11):1908-10. doi: 10.3382/ps.0741908.
An effective method for the enrichment of two cecal species of the avian Eimeria in mixed cultures has been developed. The method involves the transfer of sporozoites of Eimeria tenella and Eimeria adenoeides from their specific site of invasion in a foreign host bird to the natural host, and markedly increases the numbers of the cecal species in relation to the other species in the mixture. Donor turkeys and chickens were inoculated with mixed cultures containing equal numbers of oocysts of the chicken coccidia, E. tenella + Eimeria acervulina, or the turkey coccidia, E. adenoeides + Eimeria meleagrimitis, respectively. After 8 to 24 h, the cecal tissues of the donor birds were scraped into phosphate-buffered saline and transferred, per os, to the natural host. On Day 6 to 7 posttransfer, numerous oocysts of E. tenella and E. adenoeides were found in the ceca of the recipient chickens and turkeys, respectively. In contrast, only occasional oocysts were found in a few birds in other areas of the intestine.
已研发出一种在混合培养物中富集两种鸡盲肠艾美耳球虫的有效方法。该方法包括将柔嫩艾美耳球虫和腺状艾美耳球虫的子孢子从其在异源宿主鸟体内的特定侵入部位转移至天然宿主，相对于混合物中的其他物种，显著增加了盲肠物种的数量。分别用含有等量鸡球虫（柔嫩艾美耳球虫+堆型艾美耳球虫）或火鸡球虫（腺状艾美耳球虫+火鸡艾美耳球虫）卵囊的混合培养物接种供体火鸡和鸡。8至24小时后，将供体鸟的盲肠组织刮入磷酸盐缓冲盐水中，并经口转移至天然宿主。转移后第6至7天，分别在受体鸡和火鸡的盲肠中发现了大量的柔嫩艾美耳球虫和腺状艾美耳球虫卵囊。相比之下，在肠道其他区域的少数鸟类中仅偶尔发现卵囊。
