Augustine P C
U.S. Department of Agriculture, Agricultural Research Center, Beltsville, Maryland 20705, USA.
Avian Dis. 1996 Oct-Dec;40(4):783-7.
The effect of prior or simultaneous administration of Eimeria acervulina on cellular invasion by E. acervulina, E. tenella, or E. adenoeides was examined in vivo and/or in vitro. In chickens immunized with E. acervulina oocysts, invasion by sporozoites from a challenge inoculum of E. acervulina increased slightly over invasion in naive controls, and invasion by sporozoites of E. tenella and E. adenoeides increased by factors of 1.99 and 2.26, respectively, over that in controls. Conversely, simultaneous inoculation of E. acervulina along with E. tenella or E. adenoeides did not have a significant effect on invasion. Cultured baby hamster kidney cells that had been previously inoculated with E. acervulina sporozoites also supported significantly greater (by factors of 1.39 to 2.66) invasion by E. adenoeides sporozoites than did control cultures. The enhancement of invasion in vitro appeared to depend upon either the interval between the inoculation of cells with E. acervulina and E. adenoeides or the interval between the replating of the cells and their inoculation with E. adenoeides. There were no gross changes in the culture morphology between the E. acervulina-inoculated and noninoculated cultures. The data indicate that prior exposure to E. acervulina enhances invasion by other species of avian coccidia and, therefore, may exacerbate the infection produced by these species.
研究了预先或同时接种堆型艾美耳球虫对堆型艾美耳球虫、柔嫩艾美耳球虫或腺状艾美耳球虫细胞侵袭的影响，实验在体内和/或体外进行。在用堆型艾美耳球虫卵囊免疫的鸡中，来自堆型艾美耳球虫攻击接种物的子孢子侵袭率比未免疫对照鸡略有增加，而柔嫩艾美耳球虫和腺状艾美耳球虫的子孢子侵袭率分别比对照增加了1.99倍和2.26倍。相反，同时接种堆型艾美耳球虫与柔嫩艾美耳球虫或腺状艾美耳球虫对侵袭没有显著影响。先前接种过堆型艾美耳球虫子孢子的培养的幼仓鼠肾细胞，也比对照培养物更显著地支持腺状艾美耳球虫子孢子的侵袭（增加了1.39至2.66倍）。体外侵袭增强似乎取决于用堆型艾美耳球虫接种细胞与用腺状艾美耳球虫接种细胞之间的间隔，或者细胞传代与用腺状艾美耳球虫接种细胞之间的间隔。接种堆型艾美耳球虫的培养物和未接种的培养物之间在培养形态上没有明显变化。数据表明，预先接触堆型艾美耳球虫会增强其他禽类球虫物种的侵袭，因此可能会加剧这些物种所产生的感染。
