Roy P
Department of Biochemistry, University of Oxford.
Virology. 1996 Feb 1;216(1):1-11. doi: 10.1006/viro.1996.0028.
Orbiviruses (Reoviridae family) are complex nonenveloped RNA viruses with seven structural proteins and a RNA genome consisting of 10 variously sized double-stranded RNA segments. Significant advances in orbivirus research have been made in recent years through the use of gene manipulation techniques coupled with the baculovirus expression system. Several orbivirus proteins have yielded to crystallization and X-ray crystallographic structure determination and, when combined with the three-dimensional image reconstruction of virion particles and cores obtained by cryoelectron microscopy, considerable insight has been gained into the intricate organization and topography of the individual viral components. Formal identification of the sites of interaction has been obtained through protein-protein interaction studies on the components of the virion particle, including those that are involved in capsid assembly. Finally, a beginning of the understanding of the sequence of assembly events has also been obtained.
环状病毒（呼肠孤病毒科）是复杂的无包膜RNA病毒，有七种结构蛋白和一个由10个大小各异的双链RNA片段组成的RNA基因组。近年来，通过基因操作技术与杆状病毒表达系统相结合，环状病毒研究取得了重大进展。几种环状病毒蛋白已成功结晶并通过X射线晶体学确定了结构，再结合通过冷冻电子显微镜获得的病毒粒子和核心的三维图像重建，人们对单个病毒成分的复杂组织和拓扑结构有了相当深入的了解。通过对病毒粒子成分（包括参与衣壳组装的成分）进行蛋白质-蛋白质相互作用研究，已正式确定了相互作用位点。最后，对组装事件的顺序也有了初步了解。
