Murao K, Uyeda I, Ando Y, Kimura I, Cabauatan P Q, Koganezawa H
Department of Agrobiology and Bioresources, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Virology. 1996 Feb 1;216(1):238-40. doi: 10.1006/viro.1996.0054.
The genome segment 12 (S12) of rice dwarf phytoreovirus (RDV) isolated from the Philippines (RDV-P) and of a variant (RDV-S-6) of RDV severe strain (RDV-S) migrated abnormally slower during polyacrylamide gel electrophoresis than that of the isolate maintained at Hokkaido University (RDV-H). Nucleotide sequence analysis revealed that rearrangement had occurred in these segments, affecting the open reading frame. A polypeptide encoded by S12 (Pns12) of RDV-P had a duplication of 28 amino acids while 1/3 of the carboxyl terminus of Pns12 was deleted in RDV-S-6 by premature termination due to a frameshift. RDV-S is always present in plants infected with the RDV-S-6 variant, suggesting that Pns12 of RDV-S-6 is defective. On the other hand, Pns12 of RDV-P was expressed and appeared to be functional in infected cells in spite of the duplication, as demonstrated by immunoblot analyses using antibody raised against Pns12 expressed in Escherichia coli.
从菲律宾分离得到的水稻矮缩植物呼肠孤病毒（RDV）（RDV-P）以及RDV强毒株（RDV-S）的一个变种（RDV-S-6）的基因组片段12（S12），在聚丙烯酰胺凝胶电泳过程中的迁移速度比保存在北海道大学的分离株（RDV-H）异常缓慢。核苷酸序列分析表明，这些片段发生了重排，影响了开放阅读框。RDV-P的S12编码的多肽（Pns12）有28个氨基酸的重复，而RDV-S-6中Pns12的羧基末端的1/3由于移码导致的提前终止而缺失。RDV-S总是存在于感染了RDV-S-6变种的植物中，这表明RDV-S-6的Pns12有缺陷。另一方面，尽管RDV-P的Pns12有重复，但通过使用针对在大肠杆菌中表达的Pns12产生的抗体进行免疫印迹分析表明，它在感染细胞中表达且似乎具有功能。
