水稻矮缩植物呼肠孤病毒田间分离株的基因组异质性及基因组第12节段变异体的核苷酸序列

Genomic heterogeneity of rice dwarf phytoreovirus field isolates and nucleotide sequences of variants of genome segment 12.

作者信息

Murao K, Suda N, Uyeda I, Isogai M, Suga H, Yamada N, Kimura I, Shikata E

机构信息

Department of Agrobiology and Bioresources, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.

出版信息

J Gen Virol. 1994 Aug;75 ( Pt 8):1843-8. doi: 10.1099/0022-1317-75-8-1843.

DOI:10.1099/0022-1317-75-8-1843

PMID:8046386

Abstract

Electrophoretic profiles of the dsRNAs of field isolates of rice dwarf virus (RDV) were compared with those of an isolate maintained at Hokkaido University (RDV-H). Unexpectedly, the genomic dsRNAs of most of the field isolates showed distinct electrophoretic mobility profiles. This was the case even among isolates from the same region. Genome segment 12 (S12) from some variants migrated faster than S12 from RDV-H. These RNAs were converted to full-length cDNAs and sequenced. S12 from all the variants had the same length of 1066 nucleotides with nucleotide sequence identities of 96 to 99%. Three open reading frames previously reported were present in all the variants, and the sequence identities were 95 to 99% for P12, 98 to 100% for P12OPa, and nearly 100% for P12OPb. A comparison of the nucleotide and amino acid sequences of the variants with sequences of the RDV-H and Akita isolate showed that there are two genomic types, one represented by RDV-H and the other by the Akita isolate.

摘要

将水稻矮缩病毒（RDV）田间分离株的双链RNA（dsRNA）电泳图谱与北海道大学保存的一个分离株（RDV-H）的图谱进行了比较。出乎意料的是，大多数田间分离株的基因组dsRNA显示出明显不同的电泳迁移图谱。即使在来自同一地区的分离株中也是如此。一些变异株的基因组片段12（S12）迁移速度比RDV-H的S12快。这些RNA被转化为全长cDNA并进行测序。所有变异株的S12长度均为1066个核苷酸，核苷酸序列同一性为96%至99%。所有变异株中均存在先前报道的三个开放阅读框，P12的序列同一性为95%至99%，P12OPa为98%至100%，P12OPb几乎为100%。将变异株的核苷酸和氨基酸序列与RDV-H和秋田分离株的序列进行比较，结果表明存在两种基因组类型，一种以RDV-H为代表，另一种以秋田分离株为代表。