Etkind P, Du J, Khan A, Pillitteri J, Wiernik P H
Our Lady of Mercy Medical Center-Comprehensive Cancer Center, New York Medical College, Bronx 10466-4002, USA.
Clin Cancer Res. 2000 Apr;6(4):1273-8.
DNA sequences with very high similarity (95-98%) to the mouse mammary tumor virus (MMTV) ENV gene have been amplified by PCR in 38.5% of human breast tumors and in <2% of normal breast tissue (Wang et al., Cancer Res., 55: 5173-5179, 1995). Intrigued by these findings, which suggested an exogenous viral etiology for a certain percentage of human breast tumors, we have screened a panel of human breast tumors and normal breast tissue for the presence of MMTV-like DNA sequences. Using similar PCR procedures and stringent hybridization techniques, we have detected the presence of MMTV-like ENV gene sequences in 37% of the human breast tumors that we have analyzed. DNA sequencing has shown these sequences to be 99% homologous to the BR6 strain of MMTV and 100% homologous to the GR and C3H strains of MMTV. We have not detected these MMTV-like sequences in normal breast tissue. However, we have detected these sequences by PCR and stringent hybridization in a T-cell lymphoma of a breast cancer patient who was simultaneously diagnosed with both diseases. Our results support the possibility of an exogenous retroviral etiology for a certain percentage of human breast tumors. Our results also suggest that a similar exogenous retroviral etiology may exist for certain human T-cell lymphomas. In many inbred strains of mice, both breast cancer and T-cell lymphoma are caused by MMTV, hence, in a certain percentage of humans, one or both of these diseases may be caused by an MMTV-like retroviral entity.
在38.5%的人类乳腺肿瘤以及不到2%的正常乳腺组织中，通过聚合酶链反应（PCR）扩增出了与小鼠乳腺肿瘤病毒（MMTV）ENV基因具有高度相似性（95 - 98%）的DNA序列（Wang等人，《癌症研究》，55: 5173 - 5179, 1995）。这些发现表明一定比例的人类乳腺肿瘤存在外源性病毒病因，这引起了我们的兴趣，因此我们对一组人类乳腺肿瘤和正常乳腺组织进行了筛查，以检测是否存在MMTV样DNA序列。使用类似的PCR程序和严格的杂交技术，我们在已分析的37%的人类乳腺肿瘤中检测到了MMTV样ENV基因序列。DNA测序显示这些序列与MMTV的BR6株同源性为99%，与MMTV的GR和C3H株同源性为100%。我们在正常乳腺组织中未检测到这些MMTV样序列。然而，在一名同时被诊断患有乳腺癌和T细胞淋巴瘤的患者的T细胞淋巴瘤中，我们通过PCR和严格杂交检测到了这些序列。我们的结果支持了一定比例的人类乳腺肿瘤存在外源性逆转录病毒病因的可能性。我们的结果还表明，某些人类T细胞淋巴瘤可能存在类似的外源性逆转录病毒病因。在许多近交系小鼠中，乳腺癌和T细胞淋巴瘤均由MMTV引起，因此，在一定比例的人类中，这些疾病中的一种或两种可能由MMTV样逆转录病毒实体引起。
